Detailed information of TA system TA210045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 5577..6375
• Replicon: plasmid pBL12EC-1
• Accession: NZ_CP079776
• Organism: Escherichia coli strain BL12

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KYX59_RS00040
• Protein ID: WP_072662620.1
• Coordinates: 5577..6098 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: KYX59_RS00045
• Protein ID: WP_001711191.1
• Coordinates: 6106..6375 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX59_RS00015	696..3410	+	2715	WP_259483171.1	tail fiber protein	-
KYX59_RS00020 (KYX59_00020)	3410..3991	+	582	WP_166494821.1	tail fiber assembly protein	-
KYX59_RS00025 (KYX59_00025)	4125..4448	+	324	WP_001711185.1	hypothetical protein	-
KYX59_RS00030 (KYX59_00030)	4462..5154	+	693	WP_033546097.1	membrane protein	-
KYX59_RS00035 (KYX59_00035)	5157..5408	+	252	WP_175067820.1	hypothetical protein	-
KYX59_RS00040 (KYX59_00040)	5577..6098	-	522	WP_072662620.1	GNAT family N-acetyltransferase	Toxin
KYX59_RS00045 (KYX59_00045)	6106..6375	-	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
KYX59_RS00050 (KYX59_00050)	6695..7360	+	666	WP_023135658.1	AAA family ATPase	-
KYX59_RS00055 (KYX59_00055)	7366..7719	+	354	WP_161953766.1	hypothetical protein	-
KYX59_RS00060 (KYX59_00060)	7772..8539	-	768	WP_001711193.1	hypothetical protein	-
KYX59_RS00065 (KYX59_00065)	8822..9547	+	726	WP_033545990.1	hypothetical protein	-
KYX59_RS00070 (KYX59_00070)	9608..10948	+	1341	WP_259483151.1	DnaB-like helicase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106228	106228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19359.18 Da
• Isoelectric Point: 8.2462

>T210045 WP_072662620.1 NZ_CP079776:c6098-5577 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T210045 NZ_CP104372:c29384-28968 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT210045 WP_001711191.1 NZ_CP079776:c6375-6106 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT210045 NZ_CP104372:c29611-29381 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2