Detailed information of TA system TA210044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 20769..21361
• Replicon: plasmid pGS04EC-3
• Accession: NZ_CP079775
• Organism: Escherichia coli strain GS04

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: KYX57_RS23675
• Protein ID: WP_000520549.1
• Coordinates: 20769..21017 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: KYX57_RS23680
• Protein ID: WP_000681613.1
• Coordinates: 21014..21361 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX57_RS23645 (KYX57_23550)	17646..18161	+	516	WP_001025395.1	J domain-containing protein	-
KYX57_RS23650 (KYX57_23555)	18266..18808	+	543	WP_001083906.1	hypothetical protein	-
KYX57_RS23655 (KYX57_23560)	18805..19056	+	252	WP_001230707.1	hypothetical protein	-
KYX57_RS23660 (KYX57_23565)	19043..19270	+	228	WP_000801440.1	hypothetical protein	-
KYX57_RS23665 (KYX57_23570)	19724..20023	+	300	WP_000650309.1	hypothetical protein	-
KYX57_RS23670 (KYX57_23575)	20114..20464	+	351	WP_000854259.1	hypothetical protein	-
KYX57_RS23675 (KYX57_23580)	20769..21017	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
KYX57_RS23680 (KYX57_23585)	21014..21361	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KYX57_RS23685 (KYX57_23590)	21722..22225	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
KYX57_RS23690 (KYX57_23595)	22229..23458	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
KYX57_RS23695 (KYX57_23600)	23535..23828	+	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
KYX57_RS23700 (KYX57_23605)	23825..24040	+	216	WP_000760375.1	EexN family lipoprotein	-
KYX57_RS23705 (KYX57_23610)	24396..25016	+	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
KYX57_RS23710 (KYX57_23615)	25030..25356	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33587	33587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T210044 WP_000520549.1 NZ_CP079775:20769-21017 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T210044 NZ_CP104371:c4266624-4266406 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT210044 WP_000681613.1 NZ_CP079775:21014-21361 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT210044 NZ_CP104371:c4267024-4266650 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1