Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 18805..19270 | Replicon | plasmid pGS04EC-3 |
| Accession | NZ_CP079775 | ||
| Organism | Escherichia coli strain GS04 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9ZUY2 |
| Locus tag | KYX57_RS23655 | Protein ID | WP_001230707.1 |
| Coordinates | 18805..19056 (+) | Length | 84 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | M7CCF8 |
| Locus tag | KYX57_RS23660 | Protein ID | WP_000801440.1 |
| Coordinates | 19043..19270 (+) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KYX57_RS23630 (KYX57_23535) | 14286..14813 | - | 528 | WP_000577043.1 | DUF2726 domain-containing protein | - |
| KYX57_RS23635 (KYX57_23540) | 14948..15352 | - | 405 | WP_012421203.1 | hypothetical protein | - |
| KYX57_RS23640 (KYX57_23545) | 15365..16237 | - | 873 | WP_001398251.1 | replication initiation protein | - |
| KYX57_RS23645 (KYX57_23550) | 17646..18161 | + | 516 | WP_001025395.1 | J domain-containing protein | - |
| KYX57_RS23650 (KYX57_23555) | 18266..18808 | + | 543 | WP_001083906.1 | hypothetical protein | - |
| KYX57_RS23655 (KYX57_23560) | 18805..19056 | + | 252 | WP_001230707.1 | hypothetical protein | Toxin |
| KYX57_RS23660 (KYX57_23565) | 19043..19270 | + | 228 | WP_000801440.1 | hypothetical protein | Antitoxin |
| KYX57_RS23665 (KYX57_23570) | 19724..20023 | + | 300 | WP_000650309.1 | hypothetical protein | - |
| KYX57_RS23670 (KYX57_23575) | 20114..20464 | + | 351 | WP_000854259.1 | hypothetical protein | - |
| KYX57_RS23675 (KYX57_23580) | 20769..21017 | + | 249 | WP_000520549.1 | type II toxin-antitoxin system HicA family toxin | - |
| KYX57_RS23680 (KYX57_23585) | 21014..21361 | + | 348 | WP_000681613.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| KYX57_RS23685 (KYX57_23590) | 21722..22225 | + | 504 | WP_000781813.1 | relaxosome-coupling protein TaxA | - |
| KYX57_RS23690 (KYX57_23595) | 22229..23458 | + | 1230 | WP_001329504.1 | DNA relaxase/nickase TaxC | - |
| KYX57_RS23695 (KYX57_23600) | 23535..23828 | + | 294 | WP_000134733.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| KYX57_RS23700 (KYX57_23605) | 23825..24040 | + | 216 | WP_000760375.1 | EexN family lipoprotein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mcr-1.1 | - | 1..33587 | 33587 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 84 a.a. Molecular weight: 9751.13 Da Isoelectric Point: 8.9187
>T210043 WP_001230707.1 NZ_CP079775:18805-19056 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
Download Length: 252 bp
>T210043 NZ_CP104371:4057996-4058394 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 76 a.a. Molecular weight: 8659.98 Da Isoelectric Point: 8.5105
>AT210043 WP_000801440.1 NZ_CP079775:19043-19270 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
Download Length: 228 bp
>AT210043 NZ_CP104371:4057700-4057993 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9ZUY2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Y6U8W9 |