Detailed information of TA system TA210039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 35189..35987
• Replicon: plasmid pGS04EC-1
• Accession: NZ_CP079773
• Organism: Escherichia coli strain GS04

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KYX57_RS22695
• Protein ID: WP_072662620.1
• Coordinates: 35466..35987 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: KYX57_RS22690
• Protein ID: WP_001711191.1
• Coordinates: 35189..35458 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX57_RS22665 (KYX57_22595)	30616..31956	-	1341	WP_259483151.1	DnaB-like helicase C-terminal domain-containing protein	-
KYX57_RS22670 (KYX57_22600)	32017..32742	-	726	WP_033545990.1	hypothetical protein	-
KYX57_RS22675 (KYX57_22605)	33025..33792	+	768	WP_001711193.1	hypothetical protein	-
KYX57_RS22680 (KYX57_22610)	33845..34198	-	354	WP_161953766.1	hypothetical protein	-
KYX57_RS22685 (KYX57_22615)	34204..34869	-	666	WP_023135658.1	AAA family ATPase	-
KYX57_RS22690 (KYX57_22620)	35189..35458	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
KYX57_RS22695 (KYX57_22625)	35466..35987	+	522	WP_072662620.1	GNAT family N-acetyltransferase	Toxin
KYX57_RS22700 (KYX57_22630)	36156..36407	-	252	WP_175067820.1	hypothetical protein	-
KYX57_RS22705 (KYX57_22635)	36410..37102	-	693	WP_033546097.1	membrane protein	-
KYX57_RS22710 (KYX57_22640)	37116..37439	-	324	WP_001711185.1	hypothetical protein	-
KYX57_RS22715 (KYX57_22645)	37573..38154	-	582	WP_166494821.1	tail fiber assembly protein	-
KYX57_RS22720	38154..40868	-	2715	WP_259483171.1	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106228	106228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19359.18 Da
• Isoelectric Point: 8.2462

>T210039 WP_072662620.1 NZ_CP079773:35466-35987 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T210039 NZ_CP104371:3431038-3431211 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT210039 WP_001711191.1 NZ_CP079773:35189-35458 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT210039 NZ_CP104371:3430714-3431010 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2