Detailed information of TA system TA210038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 28612..29210
• Replicon: plasmid pGS04EC-1
• Accession: NZ_CP079773
• Organism: Escherichia coli strain GS04

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: KYX57_RS22655
• Protein ID: WP_053291281.1
• Coordinates: 28833..29210 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: KYX57_RS22650
• Protein ID: WP_001603498.1
• Coordinates: 28612..28833 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX57_RS22630 (KYX57_22560)	23618..24166	+	549	WP_166498870.1	fimbrial protein YehD	-
KYX57_RS22635 (KYX57_22565)	24222..24902	+	681	WP_032187672.1	fimbrial assembly chaperone	-
KYX57_RS22640 (KYX57_22570)	24920..27406	+	2487	WP_259483145.1	fimbrial biogenesis outer membrane usher protein	-
KYX57_RS22645 (KYX57_22575)	27417..28427	+	1011	WP_259483147.1	fimbrial protein	-
KYX57_RS22650 (KYX57_22580)	28612..28833	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KYX57_RS22655 (KYX57_22585)	28833..29210	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KYX57_RS22660 (KYX57_22590)	29344..30528	-	1185	WP_023135654.1	hypothetical protein	-
KYX57_RS22665 (KYX57_22595)	30616..31956	-	1341	WP_259483151.1	DnaB-like helicase C-terminal domain-containing protein	-
KYX57_RS22670 (KYX57_22600)	32017..32742	-	726	WP_033545990.1	hypothetical protein	-
KYX57_RS22675 (KYX57_22605)	33025..33792	+	768	WP_001711193.1	hypothetical protein	-
KYX57_RS22680 (KYX57_22610)	33845..34198	-	354	WP_161953766.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106228	106228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T210038 WP_053291281.1 NZ_CP079773:28833-29210 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T210038 NZ_CP104371:c2788122-2787937 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCGTCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT210038 WP_001603498.1 NZ_CP079773:28612-28833 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT210038 NZ_CP104371:c2787920-2787510 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACTACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0