Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4363677..4364331 Replicon chromosome
Accession NZ_CP079772
Organism Escherichia coli strain GS04

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KYX57_RS21370 Protein ID WP_000244777.1
Coordinates 4363924..4364331 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KYX57_RS21365 Protein ID WP_000354046.1
Coordinates 4363677..4363943 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX57_RS21340 (4358846) 4358846..4359589 + 744 WP_000951964.1 SDR family oxidoreductase -
KYX57_RS21345 (4359646) 4359646..4361079 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KYX57_RS21350 (4361124) 4361124..4361435 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KYX57_RS21355 (4361599) 4361599..4362258 + 660 WP_000250274.1 hemolysin III family protein -
KYX57_RS21360 (4362454) 4362454..4363434 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KYX57_RS21365 (4363677) 4363677..4363943 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KYX57_RS21370 (4363924) 4363924..4364331 + 408 WP_000244777.1 protein YgfX Toxin
KYX57_RS21375 (4364371) 4364371..4364892 - 522 WP_001055874.1 flavodoxin FldB -
KYX57_RS21380 (4365004) 4365004..4365900 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KYX57_RS21385 (4365925) 4365925..4366635 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KYX57_RS21390 (4366641) 4366641..4368374 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T210036 WP_000244777.1 NZ_CP079772:4363924-4364331 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210036 NZ_CP104371:c2255683-2255369 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210036 WP_000354046.1 NZ_CP079772:4363677-4363943 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210036 NZ_CP104371:c2255372-2254956 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACGCCCAGGCGCTGGAACTGGTCGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References