Detailed information of TA system TA210012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4233586..4234184
• Replicon: chromosome
• Accession: NZ_CP079770
• Organism: Escherichia coli strain HL15

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: KYX58_RS22390
• Protein ID: WP_053270478.1
• Coordinates: 4233807..4234184 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: KYX58_RS22385
• Protein ID: WP_001603498.1
• Coordinates: 4233586..4233807 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX58_RS22355 (4228632)	4228632..4230131	+	1500	WP_000933250.1	inorganic phosphate transporter PitB	-
KYX58_RS22360 (4230180)	4230180..4230872	-	693	WP_259553631.1	dTMP kinase	-
KYX58_RS22365 (4231046)	4231046..4231759	+	714	WP_096179607.1	dTMP kinase	-
KYX58_RS22370 (4231791)	4231791..4232342	+	552	Protein_4084	hypothetical protein	-
KYX58_RS22375 (4232398)	4232398..4233095	+	698	WP_223367350.1	IS1 family transposase	-
KYX58_RS22380 (4233120)	4233120..4233401	+	282	WP_259553636.1	fimbrial protein	-
KYX58_RS22385 (4233586)	4233586..4233807	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KYX58_RS22390 (4233807)	4233807..4234184	+	378	WP_053270478.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KYX58_RS22395 (4234294)	4234294..4234509	-	216	Protein_4089	transposase	-
KYX58_RS22400 (4234695)	4234695..4235957	-	1263	WP_076486553.1	integrase arm-type DNA-binding domain-containing protein	-
KYX58_RS22410 (4236336)	4236336..4237043	-	708	WP_000234514.1	DUF554 domain-containing protein	-
KYX58_RS22415 (4237196)	4237196..4237279	+	84	WP_211180506.1	protein YqgH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4226481..4239576	13095
	-	inside	IScluster/Tn	-	-	4232802..4234509	1707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13419.20 Da
• Isoelectric Point: 6.7260

>T210012 WP_053270478.1 NZ_CP079770:4233807-4234184 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITGLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T210012 NZ_CP104366:849097-849495 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT210012 WP_001603498.1 NZ_CP079770:4233586-4233807 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT210012 NZ_CP104366:848870-849097 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0