Detailed information of TA system TA210002

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1641362..1642197
• Replicon: chromosome
• Accession: NZ_CP079770
• Organism: Escherichia coli strain HL15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KYX58_RS09835
• Protein ID: WP_000854821.1
• Coordinates: 1641362..1641739 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KYX58_RS09840
• Protein ID: WP_024257643.1
• Coordinates: 1641829..1642197 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX58_RS09795 (1636644)	1636644..1637135	-	492	WP_001300785.1	DUF1097 domain-containing protein	-
KYX58_RS09800 (1637237)	1637237..1637791	-	555	WP_001001921.1	molecular chaperone YcdY	-
KYX58_RS09805 (1637815)	1637815..1638552	-	738	WP_000283667.1	zinc-binding phosphatase	-
KYX58_RS09810 (1638607)	1638607..1639545	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
KYX58_RS09820 (1640016)	1640016..1640858	-	843	WP_105075128.1	DUF4942 domain-containing protein	-
KYX58_RS09825 (1640943)	1640943..1641140	-	198	WP_023563519.1	DUF957 domain-containing protein	-
KYX58_RS09830 (1641168)	1641168..1641365	-	198	Protein_1652	DUF5983 family protein	-
KYX58_RS09835 (1641362)	1641362..1641739	-	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KYX58_RS09840 (1641829)	1641829..1642197	-	369	WP_024257643.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KYX58_RS09845 (1642277)	1642277..1642498	-	222	WP_000692309.1	DUF987 domain-containing protein	-
KYX58_RS09850 (1642567)	1642567..1643043	-	477	WP_259554763.1	RadC family protein	-
KYX58_RS09855 (1643059)	1643059..1643544	-	486	WP_000206660.1	antirestriction protein	-
KYX58_RS09860 (1643636)	1643636..1644454	-	819	WP_259554765.1	DUF932 domain-containing protein	-
KYX58_RS09865 (1644782)	1644782..1647166	-	2385	Protein_1659	Ag43/Cah family autotransporter adhesin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T210002 WP_000854821.1 NZ_CP079770:c1641739-1641362 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T210002 NZ_CP104362:c4037781-4037473 [Salmonella enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.33 Da
• Isoelectric Point: 6.4768

>AT210002 WP_024257643.1 NZ_CP079770:c1642197-1641829 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGQPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT210002 NZ_CP104362:c4038023-4037784 [Salmonella enterica]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54