Detailed information of TA system TA210001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1234941..1235579
• Replicon: chromosome
• Accession: NZ_CP079770
• Organism: Escherichia coli strain HL15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KYX58_RS07740
• Protein ID: WP_000813794.1
• Coordinates: 1235403..1235579 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KYX58_RS07735
• Protein ID: WP_001270286.1
• Coordinates: 1234941..1235357 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX58_RS07715 (1230093)	1230093..1231034	-	942	WP_001251304.1	ABC transporter permease	-
KYX58_RS07720 (1231035)	1231035..1232048	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KYX58_RS07725 (1232066)	1232066..1233211	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KYX58_RS07730 (1233456)	1233456..1234862	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KYX58_RS07735 (1234941)	1234941..1235357	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KYX58_RS07740 (1235403)	1235403..1235579	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KYX58_RS07745 (1235801)	1235801..1236031	+	231	WP_000494244.1	YncJ family protein	-
KYX58_RS07750 (1236123)	1236123..1238084	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KYX58_RS07755 (1238157)	1238157..1238693	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KYX58_RS07760 (1238785)	1238785..1239960	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T210001 WP_000813794.1 NZ_CP079770:c1235579-1235403 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T210001 NZ_CP104362:c3987797-3987510 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT210001 WP_001270286.1 NZ_CP079770:c1235357-1234941 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT210001 NZ_CP104362:c3987523-3987221 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 6HPB