Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/HipA-HipB |
| Location | 230925..232548 | Replicon | plasmid pHL15EC-1 |
| Accession | NZ_CP079769 | ||
| Organism | Escherichia coli strain HL15 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | H9TH67 |
| Locus tag | KYX58_RS01230 | Protein ID | WP_000088046.1 |
| Coordinates | 230925..232250 (-) | Length | 442 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | A0A5Z6V2D1 |
| Locus tag | KYX58_RS01235 | Protein ID | WP_024136327.1 |
| Coordinates | 232255..232548 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KYX58_RS01200 (KYX58_01190) | 226909..227766 | - | 858 | WP_000784387.1 | HNH endonuclease signature motif containing protein | - |
| KYX58_RS01205 (KYX58_01195) | 227782..228090 | - | 309 | WP_001224687.1 | hypothetical protein | - |
| KYX58_RS01210 (KYX58_01200) | 228639..229064 | - | 426 | WP_001043843.1 | hypothetical protein | - |
| KYX58_RS01215 (KYX58_01205) | 229318..230133 | - | 816 | WP_053276223.1 | HNH endonuclease signature motif containing protein | - |
| KYX58_RS01220 (KYX58_01210) | 230146..230556 | - | 411 | WP_000985911.1 | hypothetical protein | - |
| KYX58_RS01225 (KYX58_01215) | 230658..230864 | - | 207 | WP_000134171.1 | hypothetical protein | - |
| KYX58_RS01230 (KYX58_01220) | 230925..232250 | - | 1326 | WP_000088046.1 | type II toxin-antitoxin system HipA family toxin | Toxin |
| KYX58_RS01235 (KYX58_01225) | 232255..232548 | - | 294 | WP_024136327.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| KYX58_RS01240 (KYX58_01230) | 233152..233544 | - | 393 | WP_000341066.1 | hypothetical protein | - |
| KYX58_RS01245 (KYX58_01235) | 233917..234258 | + | 342 | WP_001151575.1 | hypothetical protein | - |
| KYX58_RS01250 (KYX58_01240) | 234350..234541 | + | 192 | WP_000398480.1 | hypothetical protein | - |
| KYX58_RS01255 (KYX58_01245) | 234591..234872 | + | 282 | WP_000077926.1 | hypothetical protein | - |
| KYX58_RS01260 | 235213..235344 | - | 132 | WP_000374059.1 | hypothetical protein | - |
| KYX58_RS01265 (KYX58_01250) | 235435..236676 | - | 1242 | WP_001053338.1 | VWA domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | oqxB / oqxA / sul1 / blaCTX-M-65 / floR / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / lnu(F) / ant(3'')-Ia / blaTEM-1B / sul2 / tet(A) / tet(M) / mph(A) / mcr-1.1 | - | 1..266941 | 266941 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 442 a.a. Molecular weight: 50249.20 Da Isoelectric Point: 6.1927
>T209990 WP_000088046.1 NZ_CP079769:c232250-230925 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
Download Length: 1326 bp
>T209990 NZ_CP104359:c56671-56366 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 10946.35 Da Isoelectric Point: 10.0925
>AT209990 WP_024136327.1 NZ_CP079769:c232548-232255 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
Download Length: 294 bp
>AT209990 NZ_CP104359:c56891-56673 [Salmonella enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3T0VBU1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Z6V2D1 |