Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2795715..2796291 Replicon chromosome
Accession NZ_CP079767
Organism Escherichia coli strain SF10

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag KX927_RS16065 Protein ID WP_001299718.1
Coordinates 2796004..2796291 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag KX927_RS16060 Protein ID WP_000063159.1
Coordinates 2795715..2796017 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KX927_RS16045 (2792304) 2792304..2794454 + 2151 WP_001299714.1 pyruvate/proton symporter BtsT -
KX927_RS16050 (2794504) 2794504..2794707 + 204 WP_000467859.1 YbdD/YjiX family protein -
KX927_RS16055 (2794718) 2794718..2795674 + 957 WP_001299717.1 GTPase -
KX927_RS16060 (2795715) 2795715..2796017 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
KX927_RS16065 (2796004) 2796004..2796291 - 288 WP_001299718.1 BrnT family toxin Toxin
KX927_RS16070 (2796488) 2796488..2797654 + 1167 Protein_2740 restriction endonuclease -
KX927_RS16075 (2797718) 2797718..2799337 + 1620 WP_001029740.1 class I SAM-dependent DNA methyltransferase -
KX927_RS16080 (2799327) 2799327..2800718 + 1392 WP_000535334.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T209981 WP_001299718.1 NZ_CP079767:c2796291-2796004 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T209981 NZ_CP104358:c2693587-2693484 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT209981 WP_000063159.1 NZ_CP079767:c2796017-2795715 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT209981 NZ_CP104358:2693482-2693627 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References