Detailed information of TA system TA209977

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1636175..1637010
• Replicon: chromosome
• Accession: NZ_CP079767
• Organism: Escherichia coli strain SF10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KX927_RS10495
• Protein ID: WP_000854821.1
• Coordinates: 1636175..1636552 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KX927_RS10500
• Protein ID: WP_024257643.1
• Coordinates: 1636642..1637010 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KX927_RS10455 (1631457)	1631457..1631948	-	492	WP_001300785.1	DUF1097 domain-containing protein	-
KX927_RS10460 (1632050)	1632050..1632604	-	555	WP_001001921.1	molecular chaperone YcdY	-
KX927_RS10465 (1632628)	1632628..1633365	-	738	WP_000283667.1	zinc-binding phosphatase	-
KX927_RS10470 (1633420)	1633420..1634358	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
KX927_RS10480 (1634829)	1634829..1635671	-	843	WP_105075128.1	DUF4942 domain-containing protein	-
KX927_RS10485 (1635756)	1635756..1635953	-	198	WP_023563519.1	DUF957 domain-containing protein	-
KX927_RS10490 (1635981)	1635981..1636178	-	198	Protein_1649	DUF5983 family protein	-
KX927_RS10495 (1636175)	1636175..1636552	-	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KX927_RS10500 (1636642)	1636642..1637010	-	369	WP_024257643.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KX927_RS10505 (1637090)	1637090..1637311	-	222	WP_000692309.1	DUF987 domain-containing protein	-
KX927_RS10510 (1637380)	1637380..1637856	-	477	WP_259554763.1	RadC family protein	-
KX927_RS10515 (1637872)	1637872..1638357	-	486	WP_000206660.1	antirestriction protein	-
KX927_RS10520 (1638449)	1638449..1639267	-	819	WP_259554765.1	DUF932 domain-containing protein	-
KX927_RS10525 (1639595)	1639595..1641979	-	2385	Protein_1656	Ag43/Cah family autotransporter adhesin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T209977 WP_000854821.1 NZ_CP079767:c1636552-1636175 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T209977 NZ_CP104358:825273-825686 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.33 Da
• Isoelectric Point: 6.4768

>AT209977 WP_024257643.1 NZ_CP079767:c1637010-1636642 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGQPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT209977 NZ_CP104358:825026-825292 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54