Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 92632..93158 Replicon plasmid pSF10EC-2
Accession NZ_CP079766
Organism Escherichia coli strain SF10

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KX927_RS01945 Protein ID WP_000323025.1
Coordinates 92632..92919 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KX927_RS01950 Protein ID WP_000534858.1
Coordinates 92919..93158 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KX927_RS01910 88229..88309 - 81 Protein_96 hypothetical protein -
KX927_RS01915 (KX927_01890) 88297..88722 + 426 Protein_97 IS3 family transposase -
KX927_RS01920 89234..89356 + 123 WP_001406634.1 hypothetical protein -
KX927_RS01925 (KX927_01895) 89395..90903 + 1509 WP_141223309.1 group II intron reverse transcriptase/maturase -
KX927_RS01930 (KX927_01900) 90984..91473 + 490 Protein_100 IS3 family transposase -
KX927_RS01935 (KX927_01905) 91479..91688 - 210 WP_072662162.1 hypothetical protein -
KX927_RS01940 (KX927_01910) 92125..92349 - 225 Protein_102 hypothetical protein -
KX927_RS01945 (KX927_01915) 92632..92919 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KX927_RS01950 (KX927_01920) 92919..93158 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KX927_RS01955 93183..93293 + 111 Protein_105 protein YdfV -
KX927_RS01960 (KX927_01925) 93500..93649 + 150 WP_000550473.1 hypothetical protein -
KX927_RS01965 (KX927_01930) 93693..94766 + 1074 WP_000557466.1 hypothetical protein -
KX927_RS01970 (KX927_01935) 94843..95547 + 705 WP_001067852.1 IS6-like element IS26 family transposase -
KX927_RS01975 (KX927_01940) 95553..96077 + 525 WP_232379235.1 Ig-like domain-containing protein -
KX927_RS01980 (KX927_01945) 96420..97124 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
KX927_RS01985 (KX927_01950) 97188..97310 + 123 Protein_111 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid floR / qnrS1 / tet(A) - 1..127890 127890
- inside IScluster/Tn - - 83139..98839 15700


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T209965 WP_000323025.1 NZ_CP079766:c92919-92632 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T209965 NZ_CP104356:1145375-1146313 [Vibrio cholerae]
ATGGATAAAGATAGCCCATACTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCCACAGAGACAGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAGTTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCACCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGAATGTTACTTGGTAGTGAAGGGATTTCGAGAGAGATTTTGGTGGGTTTTTTAACCTA
TACATTAAGTCACCCTCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCTCTAAATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCCATGATGTTAATTGAACTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTCAAAAGAGGACAACCTGACTGGGCATTGTTTGACTATCCTAA
AGCAGCAGACTTGCCAGCGATTCGTTGGAAGTTGCAGAACATTAACAAATTGGCAAAGAATCAAGCAAAACATCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT209965 WP_000534858.1 NZ_CP079766:c93158-92919 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT209965 NZ_CP104356:1144591-1145382 [Vibrio cholerae]
ATGTCATCTAAGATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
CGTAAGCTACTCGTTGGCTCAGAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTTAGTTCTGGTGTGTACTATCGTCCAA
ATGCGCAAGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTAGATGTGCAATTGGGCGTTTCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATCTACAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAACAAGTCGTCCTTACCAAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGTGGAAACCATAAGCTTGAAG
TGAATCCCGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAGCTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGACGCGATTGGGAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTCTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAGCGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTAAATTGGGTGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References