Detailed information of TA system TA209964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 30918..31519
• Replicon: plasmid pSF10EC-2
• Accession: NZ_CP079766
• Organism: Escherichia coli strain SF10

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: KX927_RS01620
• Protein ID: WP_259559996.1
• Coordinates: 30918..31298 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KX927_RS01625
• Protein ID: WP_001190712.1
• Coordinates: 31298..31519 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KX927_RS01585 (KX927_01570)	26132..26353	-	222	WP_000542385.1	hypothetical protein	-
KX927_RS01590 (KX927_01575)	26958..27167	+	210	WP_032195054.1	hypothetical protein	-
KX927_RS01595 (KX927_01580)	27278..28129	+	852	WP_000611666.1	phage repressor protein C1	-
KX927_RS01600 (KX927_01585)	28162..29028	-	867	WP_259559990.1	phage antirepressor KilAC domain-containing protein	-
KX927_RS01605 (KX927_01590)	29152..29496	-	345	WP_259559992.1	Late promoter-activating protein	-
KX927_RS01610 (KX927_01595)	29663..30706	-	1044	WP_259559994.1	DUF968 domain-containing protein	-
KX927_RS01615 (KX927_01600)	30734..30913	-	180	WP_000113019.1	hypothetical protein	-
KX927_RS01620 (KX927_01605)	30918..31298	-	381	WP_259559996.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KX927_RS01625 (KX927_01610)	31298..31519	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KX927_RS01630 (KX927_01615)	31702..33258	+	1557	WP_259560012.1	type I restriction-modification system subunit M	-
KX927_RS01635 (KX927_01620)	33255..34469	+	1215	Protein_41	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	floR / qnrS1 / tet(A)	-	1..127890	127890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13671.47 Da
• Isoelectric Point: 6.2283

>T209964 WP_259559996.1 NZ_CP079766:c31298-30918 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNRVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T209964 NZ_CP104356:c1140084-1139761 [Vibrio cholerae]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGATCAGATCCTCGATATGCCACCCAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATTGGTAGAGGCTTATTTTGCTACTTAAAAGGGAAGCACATTTATGTGTTACAC
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTCAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT209964 WP_001190712.1 NZ_CP079766:c31519-31298 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT209964 NZ_CP104356:c1139764-1139486 [Vibrio cholerae]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGAAGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGATCAATTGCTTACCATGAGAACAAAGGCAGGACTTACTCAAGAGCAGGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGCAATGCCAATCCAAGTTGGTCAACTCTTCTAAAATATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6