Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 70448..72071 Replicon plasmid pSF10EC-1
Accession NZ_CP079765
Organism Escherichia coli strain SF10

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KX927_RS00425 Protein ID WP_000088046.1
Coordinates 70448..71773 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KX927_RS00430 Protein ID WP_024136327.1
Coordinates 71778..72071 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KX927_RS00395 (KX927_00390) 66432..67289 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KX927_RS00400 (KX927_00395) 67305..67613 - 309 WP_001224687.1 hypothetical protein -
KX927_RS00405 (KX927_00400) 68162..68587 - 426 WP_001043843.1 hypothetical protein -
KX927_RS00410 (KX927_00405) 68841..69656 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
KX927_RS00415 (KX927_00410) 69669..70079 - 411 WP_000985911.1 hypothetical protein -
KX927_RS00420 (KX927_00415) 70181..70387 - 207 WP_000134171.1 hypothetical protein -
KX927_RS00425 (KX927_00420) 70448..71773 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KX927_RS00430 (KX927_00425) 71778..72071 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KX927_RS00435 (KX927_00430) 72675..73067 - 393 WP_000341066.1 hypothetical protein -
KX927_RS00440 (KX927_00435) 73440..73781 + 342 WP_001151575.1 hypothetical protein -
KX927_RS00445 (KX927_00440) 73873..74064 + 192 WP_000398480.1 hypothetical protein -
KX927_RS00450 (KX927_00445) 74114..74395 + 282 WP_000077926.1 hypothetical protein -
KX927_RS00455 74736..74867 - 132 WP_000374059.1 hypothetical protein -
KX927_RS00460 (KX927_00450) 74958..76199 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / lnu(F) / ant(3'')-Ia / blaTEM-1B / sul2 / tet(A) / tet(M) / mph(A) / mcr-1.1 / oqxB / oqxA / sul1 / blaCTX-M-65 - 1..265589 265589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T209963 WP_000088046.1 NZ_CP079765:c71773-70448 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T209963 NZ_CP104355:661968-662369 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCGACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT209963 WP_024136327.1 NZ_CP079765:c72071-71778 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT209963 NZ_CP104355:661798-661968 [Vibrio cholerae]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGCACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References