Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4835400..4836019 Replicon chromosome
Accession NZ_CP079762
Organism Klebsiella pneumoniae strain JP20

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KYE76_RS23460 Protein ID WP_002892050.1
Coordinates 4835400..4835618 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KYE76_RS23465 Protein ID WP_002892066.1
Coordinates 4835645..4836019 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYE76_RS23425 (4831447) 4831447..4831710 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KYE76_RS23430 (4831710) 4831710..4831850 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KYE76_RS23435 (4831847) 4831847..4832545 - 699 WP_002892021.1 GNAT family protein -
KYE76_RS23440 (4832646) 4832646..4834097 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KYE76_RS23445 (4834072) 4834072..4834542 - 471 WP_002892026.1 YlaC family protein -
KYE76_RS23450 (4834563) 4834563..4834703 + 141 WP_004147370.1 hypothetical protein -
KYE76_RS23455 (4834675) 4834675..4835241 - 567 WP_002892030.1 maltose O-acetyltransferase -
KYE76_RS23460 (4835400) 4835400..4835618 - 219 WP_002892050.1 HHA domain-containing protein Toxin
KYE76_RS23465 (4835645) 4835645..4836019 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KYE76_RS23470 (4836505) 4836505..4839651 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KYE76_RS23475 (4839674) 4839674..4840867 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209961 WP_002892050.1 NZ_CP079762:c4835618-4835400 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209961 NZ_CP104355:655464-655766 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCCGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGATT
AGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209961 WP_002892066.1 NZ_CP079762:c4836019-4835645 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209961 NZ_CP104355:655219-655476 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
TTTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References