Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4196520..4197330 Replicon chromosome
Accession NZ_CP079762
Organism Klebsiella pneumoniae strain JP20

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KYE76_RS20435 Protein ID WP_004178461.1
Coordinates 4196797..4197330 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KYE76_RS20430 Protein ID WP_002887278.1
Coordinates 4196520..4196786 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYE76_RS20410 (4192392) 4192392..4192736 - 345 WP_004197273.1 cation efflux system protein CusF -
KYE76_RS20415 (4192754) 4192754..4194139 - 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KYE76_RS20420 (4194311) 4194311..4194994 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
KYE76_RS20425 (4194984) 4194984..4196417 + 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KYE76_RS20430 (4196520) 4196520..4196786 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KYE76_RS20435 (4196797) 4196797..4197330 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KYE76_RS20440 (4197378) 4197378..4198499 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209960 WP_004178461.1 NZ_CP079762:4196797-4197330 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209960 NZ_CP104355:650006-650272 [Vibrio cholerae]
ATGATTAAGTTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
GTTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCGGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209960 WP_002887278.1 NZ_CP079762:4196520-4196786 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209960 NZ_CP104355:650259-650501 [Vibrio cholerae]
ATGAAAGCGCATGAGTTTGATGCCAAGTTTGAAAGTGACGACGATATCGTAATGGACTTGGATCTATCGCAAGCTAAAAG
ACCGATGCATAAGCAAAAACGAGTCAATGTGGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTCGTA
TTGGTGTAACACGCCAATCAATCATTAAAATTTGGTTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTGAGA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References