Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3636922..3637547 Replicon chromosome
Accession NZ_CP079762
Organism Klebsiella pneumoniae strain JP20

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KYE76_RS17785 Protein ID WP_002882817.1
Coordinates 3637164..3637547 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KYE76_RS17780 Protein ID WP_004150355.1
Coordinates 3636922..3637164 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYE76_RS17755 (3632913) 3632913..3633512 + 600 WP_004151865.1 glucose-1-phosphatase -
KYE76_RS17760 (3633506) 3633506..3634366 + 861 WP_004146232.1 virulence factor BrkB family protein -
KYE76_RS17765 (3634363) 3634363..3634800 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KYE76_RS17770 (3634845) 3634845..3635786 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KYE76_RS17775 (3635800) 3635800..3636717 - 918 WP_004181612.1 alpha/beta hydrolase -
KYE76_RS17780 (3636922) 3636922..3637164 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KYE76_RS17785 (3637164) 3637164..3637547 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KYE76_RS17790 (3637721) 3637721..3638650 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KYE76_RS17795 (3638647) 3638647..3639282 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KYE76_RS17800 (3639279) 3639279..3640181 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209957 WP_002882817.1 NZ_CP079762:3637164-3637547 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209957 NZ_CP104355:645169-645471 [Vibrio cholerae]
ATGGCTGAAATAGTTTGGACGGAACCAGCTCTATCCGATCTAAATGATATTGCTGAATACATAGCGCTTGAAAATGTCGT
AGCTGCTAAACAACTGGTGCAAACTGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGACGCATTCCAC
CAGAACTAGAGCACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGCCGTGTTTTCTACAAGTATGATGATGCAAATGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209957 WP_004150355.1 NZ_CP079762:3636922-3637164 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209957 NZ_CP104355:644924-645181 [Vibrio cholerae]
ATGAAAGTAGAACTTGTAACGTCACTAAAACGGCAGGCCACTAAGATCCTTGCCGATCTTCATGATACTAAAGAGCCAGT
ATTGATAACTGAGCATGGAAAGCCGTCAGCTTATCTTATTGACGTAGATGATTATGAATTTATGCAAAATCGTTTAGCAA
TCCTCGAAGGAATTGCTCGTGGCGAGCGAGCTCTAGCGGATGGCAAAGTCGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGCTGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References