Detailed information of TA system TA020995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 990571..991354
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q7N879
• Locus tag: PLU_RS04295
• Protein ID: WP_011145230.1
• Coordinates: 990854..991354 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: PLU_RS04290
• Protein ID: WP_011145229.1
• Coordinates: 990571..990849 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS04265 (plu0856)	985888..987054	-	1167	WP_011145224.1	cupin domain-containing protein	-
PLU_RS04270 (plu0857)	987214..987630	-	417	WP_192880152.1	hypothetical protein	-
PLU_RS04280 (plu0859)	989172..989435	+	264	WP_011145227.1	helix-turn-helix domain-containing protein	-
PLU_RS04285 (plu0860)	989805..990350	+	546	WP_011145228.1	hypoxanthine phosphoribosyltransferase	-
PLU_RS04290 (plu0861)	990571..990849	+	279	WP_011145229.1	DUF1778 domain-containing protein	Antitoxin
PLU_RS04295 (plu0862)	990854..991354	+	501	WP_011145230.1	GNAT family N-acetyltransferase	Toxin
PLU_RS04300 (plu0863)	991509..992048	+	540	WP_041379935.1	SocA family protein	-
PLU_RS04305 (plu0864)	992383..992919	+	537	WP_011145232.1	hypoxanthine phosphoribosyltransferase	-
PLU_RS04310 (plu0865)	993019..993297	-	279	WP_011145233.1	HigA family addiction module antidote protein	-
PLU_RS04315 (plu0866)	993308..993589	-	282	WP_011145234.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PLU_RS04320 (plu0867)	995109..995762	-	654	WP_011145235.1	carbonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-92)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18532.23 Da
• Isoelectric Point: 9.2491

>T20995 WP_011145230.1 NC_005126:990854-991354 [Photorhabdus laumondii subsp. laumondii TTO1]
MINDPTLLTDDHNLNSFTSGIDTLDNWLTDRALKNQKENASRTYVVSEDRDVIAYYSLATGSIMSEEAISKFKRNMPNPI
PTIILGRLAVDKRYQGRGFGRYLVRDASIRVAQAADLVGVRGLIVHAISENAKAFYKSVGFSDSLLNPMTLMITLKDLKT
VFKITD

Nucleotide

• Length: 501 bp

>T20995 NC_005126:990854-991354 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGATTAATGATCCAACCTTGTTAACAGATGATCACAATTTAAATAGTTTTACTTCAGGAATTGATACCCTAGACAACTG
GTTAACAGACAGAGCATTAAAAAATCAAAAAGAGAATGCTTCACGAACCTATGTTGTAAGCGAAGACAGAGATGTTATTG
CTTATTATTCGTTAGCGACCGGCTCAATTATGTCAGAAGAGGCGATAAGCAAGTTTAAGCGTAATATGCCAAATCCTATT
CCGACGATTATTTTGGGTCGCTTAGCTGTTGATAAGCGTTATCAAGGAAGAGGGTTTGGTCGCTATTTAGTTAGAGATGC
TTCCATACGTGTCGCTCAAGCGGCAGATTTGGTGGGTGTTAGGGGGTTGATTGTCCATGCTATCTCGGAGAATGCTAAGG
CATTTTATAAGTCTGTGGGTTTTAGTGATTCGCTTTTAAATCCAATGACGTTAATGATCACGCTAAAGGATTTAAAAACG
GTTTTTAAAATAACAGATTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10637.37 Da
• Isoelectric Point: 10.2880

>AT20995 WP_011145229.1 NC_005126:990571-990849 [Photorhabdus laumondii subsp. laumondii TTO1]
MNVISENRKNKTLNLRIRQEDRDLIDRAAKVKGKTVTEYVLDTIKRDAENTLLEHSFMIVSPEIFNAFIAKLDAPAVPNE
RLIKTANMKKPW

Nucleotide

• Length: 279 bp

>AT20995 NC_005126:990571-990849 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGAACGTAATAAGTGAGAATCGTAAAAATAAGACACTCAACTTGAGGATTAGGCAAGAGGATCGGGATTTAATAGATAG
AGCAGCAAAAGTGAAGGGAAAAACCGTTACTGAATATGTCCTTGATACAATCAAACGTGATGCTGAAAACACGTTATTGG
AACATTCATTTATGATTGTATCTCCAGAAATTTTTAATGCTTTTATTGCTAAACTTGATGCTCCGGCTGTTCCAAATGAG
CGTCTTATCAAAACGGCAAATATGAAAAAACCGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A6L9JXS3