Detailed information of TA system TA209933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 507128..507764
• Replicon: chromosome
• Accession: NZ_CP079759
• Organism: Bacillus sp. LJBS17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KXZ66_RS02570
• Protein ID: WP_003156187.1
• Coordinates: 507414..507764 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: KXZ66_RS02565
• Protein ID: WP_003225183.1
• Coordinates: 507128..507409 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXZ66_RS02545 (503487)	503487..504086	-	600	WP_219144775.1	rhomboid family intramembrane serine protease	-
KXZ66_RS02550 (504181)	504181..504546	+	366	WP_015252768.1	holo-ACP synthase	-
KXZ66_RS02555 (504712)	504712..505728	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
KXZ66_RS02560 (505843)	505843..507012	+	1170	WP_219144776.1	alanine racemase	-
KXZ66_RS02565 (507128)	507128..507409	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KXZ66_RS02570 (507414)	507414..507764	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KXZ66_RS02575 (507879)	507879..508703	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
KXZ66_RS02580 (508708)	508708..509073	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
KXZ66_RS02585 (509077)	509077..509478	+	402	WP_003225192.1	serine/threonine-protein kinase RsbT	-
KXZ66_RS02590 (509490)	509490..510497	+	1008	WP_014478900.1	phosphoserine phosphatase RsbU	-
KXZ66_RS02595 (510566)	510566..510895	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
KXZ66_RS02600 (510892)	510892..511374	+	483	WP_021481702.1	anti-sigma B factor RsbW	-
KXZ66_RS02605 (511340)	511340..512128	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
KXZ66_RS02610 (512128)	512128..512727	+	600	WP_021481701.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T209933 WP_003156187.1 NZ_CP079759:507414-507764 [Bacillus sp. LJBS17]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T209933 NZ_CP104345:145981-146268 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT209933 WP_003225183.1 NZ_CP079759:507128-507409 [Bacillus sp. LJBS17]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT209933 NZ_CP104345:146255-146569 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32