Detailed information of TA system TA209918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 113965..114701
• Replicon: plasmid pNY1A
• Accession: NZ_CP079753
• Organism: Raoultella ornithinolytica strain NY1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: KXJ76_RS26915
• Protein ID: WP_003026803.1
• Coordinates: 113965..114447 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KXJ76_RS26920
• Protein ID: WP_003026799.1
• Coordinates: 114435..114701 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ76_RS26900 (KXJ76_26900)	110058..111038	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
KXJ76_RS26905 (KXJ76_26905)	111154..111906	+	753	Protein_121	IS3-like element ISKpn38 family transposase	-
KXJ76_RS27475	112115..112246	-	132	WP_004218042.1	hypothetical protein	-
KXJ76_RS26910 (KXJ76_26910)	112407..113753	-	1347	WP_020314316.1	ISNCY family transposase	-
KXJ76_RS26915 (KXJ76_26915)	113965..114447	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
KXJ76_RS26920 (KXJ76_26920)	114435..114701	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KXJ76_RS26925 (KXJ76_26925)	114971..115759	+	789	WP_077255422.1	hypothetical protein	-
KXJ76_RS26930 (KXJ76_26930)	115705..116409	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KXJ76_RS26935 (KXJ76_26935)	116503..117003	-	501	WP_003100856.1	hypothetical protein	-
KXJ76_RS26940 (KXJ76_26940)	117000..117326	-	327	WP_006812576.1	hypothetical protein	-
KXJ76_RS26945 (KXJ76_26945)	117581..117937	-	357	WP_003465043.1	cupin domain-containing protein	-
KXJ76_RS26950 (KXJ76_26950)	117927..118328	-	402	WP_001293886.1	DUF86 domain-containing protein	-
KXJ76_RS26955 (KXJ76_26955)	118325..118615	-	291	WP_001247892.1	nucleotidyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..167012	167012
	-	inside	IScluster/Tn	-	-	110058..131693	21635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T209918 WP_003026803.1 NZ_CP079753:c114447-113965 [Raoultella ornithinolytica]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T209918 NZ_CP104336:c293877-293590 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT209918 WP_003026799.1 NZ_CP079753:c114701-114435 [Raoultella ornithinolytica]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT209918 NZ_CP104336:c293603-293289 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66