Detailed information of TA system TA209915

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5167523..5168295
• Replicon: chromosome
• Accession: NZ_CP079752
• Organism: Raoultella ornithinolytica strain NY1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A443UJW7
• Locus tag: KXJ76_RS24360
• Protein ID: WP_065523303.1
• Coordinates: 5167906..5168295 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KXJ76_RS24355
• Protein ID: WP_176451017.1
• Coordinates: 5167523..5167849 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ76_RS24325 (KXJ76_24325)	5163968..5164600	-	633	WP_219173358.1	inovirus Gp2 family protein	-
KXJ76_RS24330 (KXJ76_24330)	5165138..5165962	+	825	WP_049594226.1	DUF932 domain-containing protein	-
KXJ76_RS24335 (KXJ76_24335)	5165986..5166216	+	231	WP_094963399.1	DUF905 domain-containing protein	-
KXJ76_RS24340 (KXJ76_24340)	5166296..5166739	+	444	WP_049594225.1	antirestriction protein	-
KXJ76_RS24345 (KXJ76_24345)	5166751..5167230	+	480	WP_094963398.1	DNA repair protein RadC	-
KXJ76_RS24350 (KXJ76_24350)	5167244..5167465	+	222	WP_047724679.1	DUF987 domain-containing protein	-
KXJ76_RS24355 (KXJ76_24355)	5167523..5167849	+	327	WP_176451017.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KXJ76_RS24360 (KXJ76_24360)	5167906..5168295	+	390	WP_065523303.1	TA system toxin CbtA family protein	Toxin
KXJ76_RS24365 (KXJ76_24365)	5168368..5169212	+	845	Protein_4799	DUF4942 domain-containing protein	-
KXJ76_RS27340	5170614..5170791	-	178	Protein_4800	IS1 family transposase	-
KXJ76_RS24375 (KXJ76_24375)	5171236..5172546	+	1311	WP_064359448.1	L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14411.53 Da
• Isoelectric Point: 9.8627

>T209915 WP_065523303.1 NZ_CP079752:5167906-5168295 [Raoultella ornithinolytica]
MQTKSSPLMRAASSRPSPVYIWQTLLTSLLAQHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T209915 NZ_CP104336:c290208-289888 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12195.94 Da
• Isoelectric Point: 6.9557

>AT209915 WP_176451017.1 NZ_CP079752:5167523-5167849 [Raoultella ornithinolytica]
ISEPWWGLRRSVSPCFGARLVQERNRLHYLADRANFDGQFCDADLRHLDQAFPVLLKQLELMLTSGELNPRHQHGIILYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT209915 NZ_CP104336:c289895-289623 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A443UJW7