Detailed information of TA system TA209914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4830408..4830987
• Replicon: chromosome
• Accession: NZ_CP079752
• Organism: Raoultella ornithinolytica strain NY1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A808YBH6
• Locus tag: KXJ76_RS22820
• Protein ID: WP_032716505.1
• Coordinates: 4830700..4830987 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: KXJ76_RS22815
• Protein ID: WP_004857518.1
• Coordinates: 4830408..4830713 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ76_RS22785 (KXJ76_22780)	4825449..4825703	+	255	WP_004857528.1	DUF3343 domain-containing protein	-
KXJ76_RS22790 (KXJ76_22785)	4825700..4826764	-	1065	WP_004857527.1	DUF2955 domain-containing protein	-
KXJ76_RS22795 (KXJ76_22790)	4826754..4827821	-	1068	WP_004857525.1	HlyD family secretion protein	-
KXJ76_RS22800 (KXJ76_22795)	4827828..4828292	-	465	WP_004857523.1	MarR family transcriptional regulator	-
KXJ76_RS22805 (KXJ76_22800)	4828499..4828663	-	165	WP_004857521.1	DUF1127 domain-containing protein	-
KXJ76_RS22810 (KXJ76_22805)	4828841..4830253	+	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
KXJ76_RS22815 (KXJ76_22810)	4830408..4830713	-	306	WP_004857518.1	BrnA antitoxin family protein	Antitoxin
KXJ76_RS22820 (KXJ76_22815)	4830700..4830987	-	288	WP_032716505.1	BrnT family toxin	Toxin
KXJ76_RS22825 (KXJ76_22820)	4831105..4831524	-	420	WP_015585224.1	FosA family fosfomycin resistance glutathione transferase	-
KXJ76_RS22830 (KXJ76_22825)	4831566..4832426	-	861	WP_219173332.1	LysR family transcriptional regulator	-
KXJ76_RS22835 (KXJ76_22830)	4832514..4833296	+	783	WP_123629037.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11131.57 Da
• Isoelectric Point: 7.5235

>T209914 WP_032716505.1 NZ_CP079752:c4830987-4830700 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T209914 NZ_CP104335:c4089033-4088851 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11681.22 Da
• Isoelectric Point: 9.4429

>AT209914 WP_004857518.1 NZ_CP079752:c4830713-4830408 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIREQNKKS

Nucleotide

• Length: 306 bp

>AT209914 NZ_CP104335:c4088758-4088354 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: A0A808YBH6