Detailed information of TA system TA020991

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 143078..143766
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: PLU_RS00680
• Protein ID: WP_011144541.1
• Coordinates: 143479..143766 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: Q7NA13
• Locus tag: PLU_RS00675
• Protein ID: WP_011144540.1
• Coordinates: 143078..143404 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS00650 (plu0128)	138836..139230	-	395	Protein_126	VOC family protein	-
PLU_RS24795	139425..139592	+	168	WP_082302939.1	DNA alkylation repair protein	-
PLU_RS00655 (plu0130)	139636..140232	-	597	WP_011144536.1	hypothetical protein	-
PLU_RS00660 (plu0131)	140512..141642	+	1131	WP_011144537.1	ISAs1-like element ISPlu17 family transposase	-
PLU_RS00665	141951..142424	+	474	WP_041379856.1	hypothetical protein	-
PLU_RS00670 (plu0133)	142562..143035	+	474	WP_011144539.1	DNA repair protein RadC	-
PLU_RS00675 (plu0134)	143078..143404	+	327	WP_011144540.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PLU_RS00680 (plu0135)	143479..143766	+	288	WP_011144541.1	TA system toxin CbtA family protein	Toxin
PLU_RS00685 (plu0136)	143880..144713	+	834	WP_011144542.1	DUF4942 domain-containing protein	-
PLU_RS00690 (plu0137)	144801..145838	+	1038	WP_011144543.1	virulence RhuM family protein	-
PLU_RS24800 (plu0138)	145968..146102	+	135	Protein_136	virulence RhuM family protein	-
PLU_RS26275	146354..146524	+	171	WP_157852154.1	hypothetical protein	-
PLU_RS00695 (plu0139)	146503..147273	-	771	WP_011144544.1	IclR family transcriptional regulator	-
PLU_RS00700 (plu0140)	147418..147918	+	501	WP_011144545.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-90)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11027.57 Da
• Isoelectric Point: 6.8689

>T20991 WP_011144541.1 NC_005126:143479-143766 [Photorhabdus laumondii subsp. laumondii TTO1]
VLVWQTLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLADAVNFLVEKYELVRIDLRRFNCQEQSPYLRAVDILRAR
QATGLLRQSHHPSTR

Nucleotide

• Length: 288 bp

>T20991 NC_005126:143479-143766 [Photorhabdus laumondii subsp. laumondii TTO1]
GTGCTTGTCTGGCAAACTCTACTTACACGTCTGCTGGAGCAACATTATGGCCTCACCCTGAATGACACGCCGTTCAGCGA
TGAAACTGTTATTCAGGAACATATCAACGCTGGTATTACATTGGCTGATGCCGTTAACTTTTTGGTAGAAAAGTACGAAC
TGGTTCGTATCGACCTCAGGAGGTTTAACTGTCAGGAGCAATCACCTTATCTTCGGGCTGTAGATATTTTACGGGCAAGG
CAAGCAACCGGCCTTTTGCGGCAAAGCCATCATCCTTCAACTCGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12522.12 Da
• Isoelectric Point: 5.5236

>AT20991 WP_011144540.1 NC_005126:143078-143404 [Photorhabdus laumondii subsp. laumondii TTO1]
MEKNDNPEWGLRYDLMPRFTARLVQEGNRLHYLADRAGITGTFNEEECHQLNAAFPHFIKQMELMLLSGELNSRDAHRVT
LYHNELTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT20991 NC_005126:143078-143404 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGGAAAAAAACGATAACCCTGAATGGGGGCTTCGGTACGATTTAATGCCTCGCTTTACCGCAAGGTTAGTACAGGAAGG
CAATCGACTACATTATTTAGCTGACCGCGCCGGGATAACTGGCACTTTCAATGAGGAAGAGTGCCACCAACTGAATGCCG
CATTTCCACACTTTATTAAACAGATGGAGCTGATGCTGCTCTCTGGTGAGCTTAACTCCCGCGATGCACACCGTGTAACG
CTGTACCACAACGAACTGACCTGTGAAGCGGATACGCTCGGTAGCTGTGGCTACGTTTACATCGCTATCTATCCCACTCA
ACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6L9JL04