Detailed information of TA system TA209900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 67083..67630
• Replicon: chromosome
• Accession: NZ_CP079752
• Organism: Raoultella ornithinolytica strain NY1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: KXJ76_RS00315
• Protein ID: WP_123629131.1
• Coordinates: 67083..67391 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A808XYT1
• Locus tag: KXJ76_RS00320
• Protein ID: WP_004869059.1
• Coordinates: 67394..67630 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ76_RS00285 (KXJ76_00285)	62262..62564	-	303	WP_015585486.1	PTS lactose/cellobiose transporter subunit IIA	-
KXJ76_RS00290 (KXJ76_00290)	62699..64024	-	1326	WP_004869064.1	PTS cellobiose transporter subunit IIC	-
KXJ76_RS00295 (KXJ76_00295)	64036..64350	-	315	WP_004869063.1	PTS sugar transporter subunit IIB	-
KXJ76_RS00300 (KXJ76_00300)	64645..65586	+	942	WP_015585487.1	LacI family DNA-binding transcriptional regulator	-
KXJ76_RS00305 (KXJ76_00305)	65681..65956	-	276	WP_160873538.1	YicS family protein	-
KXJ76_RS00310 (KXJ76_00310)	66065..66967	-	903	WP_015585496.1	EamA family transporter	-
KXJ76_RS00315 (KXJ76_00315)	67083..67391	-	309	WP_123629131.1	CcdB family protein	Toxin
KXJ76_RS00320 (KXJ76_00320)	67394..67630	-	237	WP_004869059.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
KXJ76_RS00325 (KXJ76_00325)	67736..69169	-	1434	WP_069476354.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
KXJ76_RS00330 (KXJ76_00330)	69194..70096	-	903	WP_123629130.1	N-acetylmuramic acid 6-phosphate etherase	-
KXJ76_RS00335 (KXJ76_00335)	70261..71424	-	1164	WP_004869055.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11792.64 Da
• Isoelectric Point: 6.7156

>T209900 WP_123629131.1 NZ_CP079752:c67391-67083 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRIVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Nucleotide

• Length: 309 bp

>T209900 NZ_CP104330:3400110-3400328 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8898.08 Da
• Isoelectric Point: 5.6579

>AT209900 WP_004869059.1 NZ_CP079752:c67630-67394 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT209900 NZ_CP104330:3399710-3400084 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A808XYT1