Detailed information of TA system TA209897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1488496..1490210
• Replicon: chromosome
• Accession: NZ_CP079751
• Organism: Aeromonas sanarellii strain NS1

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: KXJ75_RS07200
• Protein ID: WP_219143813.1
• Coordinates: 1488496..1489149 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: KXJ75_RS07205
• Protein ID: WP_219143814.1
• Coordinates: 1489146..1490210 (+)
• Length: 355 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ75_RS07180 (KXJ75_07180)	1484841..1485575	+	735	WP_219143811.1	restriction endonuclease subunit S	-
KXJ75_RS07185 (KXJ75_07185)	1485628..1486578	+	951	WP_182927747.1	IS1595-like element ISAeca5 family transposase	-
KXJ75_RS07190 (KXJ75_07190)	1486629..1487069	+	441	WP_219143812.1	restriction endonuclease subunit S	-
KXJ75_RS07195 (KXJ75_07195)	1487136..1488086	+	951	WP_182927747.1	IS1595-like element ISAeca5 family transposase	-
KXJ75_RS07200 (KXJ75_07200)	1488496..1489149	+	654	WP_219143813.1	DUF4433 domain-containing protein	Toxin
KXJ75_RS07205 (KXJ75_07205)	1489146..1490210	+	1065	WP_219143814.1	macro domain-containing protein	Antitoxin
KXJ75_RS07210 (KXJ75_07210)	1490285..1493416	+	3132	WP_219143815.1	HsdR family type I site-specific deoxyribonuclease	-
KXJ75_RS07215 (KXJ75_07215)	1493416..1494129	+	714	WP_005331303.1	SprT family zinc-dependent metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1479625..1606015	126390
	-	flank	IS/Tn	-	-	1494332..1495282	950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25333.93 Da
• Isoelectric Point: 7.0171

>T209897 WP_219143813.1 NZ_CP079751:1488496-1489149 [Aeromonas sanarellii]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQNPHWVHIGLPELIAKRARHPVPLAPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPRRENDEILILVSSLHRLAELGLPFVFTDSHAYYQWANFYVDLGDLGRIDWPLLQRRDFKRDADDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNSVEQCMYRVGVARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

>T209897 NZ_CP104330:2608693-2608800 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 355 a.a.
• Molecular weight: 39545.67 Da
• Isoelectric Point: 6.1445

>AT209897 WP_219143814.1 NZ_CP079751:1489146-1490210 [Aeromonas sanarellii]
MILYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQDGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTLSALREGL
HRWPAGTPWGERKARLFDDRSLQIALTRLQQGLV

Nucleotide

• Length: 1065 bp

>AT209897 NZ_CP104330:c2608646-2608580 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT