Detailed information of TA system TA209896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 42372..42960
• Replicon: plasmid pMRA
• Accession: NZ_CP079749
• Organism: Acinetobacter johnsonii strain MR1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: KXJ74_RS17675
• Protein ID: WP_107010192.1
• Coordinates: 42673..42960 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: KXJ74_RS17670
• Protein ID: WP_107010193.1
• Coordinates: 42372..42686 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ74_RS17645 (KXJ74_17645)	37594..38964	+	1371	WP_219191768.1	SIR2 family protein	-
KXJ74_RS17650 (KXJ74_17650)	39379..39780	+	402	WP_219191769.1	hypothetical protein	-
KXJ74_RS17655 (KXJ74_17655)	39777..40295	+	519	WP_228738031.1	hypothetical protein	-
KXJ74_RS17660 (KXJ74_17660)	40636..41247	+	612	WP_077169897.1	recombinase family protein	-
KXJ74_RS17665 (KXJ74_17665)	41421..41801	-	381	WP_107010194.1	PAS domain S-box protein	-
KXJ74_RS18450	42140..42334	-	195	Protein_47	hypothetical protein	-
KXJ74_RS17670 (KXJ74_17670)	42372..42686	-	315	WP_107010193.1	BrnA antitoxin family protein	Antitoxin
KXJ74_RS17675 (KXJ74_17675)	42673..42960	-	288	WP_107010192.1	BrnT family toxin	Toxin
KXJ74_RS17680 (KXJ74_17680)	43216..44539	-	1324	Protein_50	ISNCY family transposase	-
KXJ74_RS17685 (KXJ74_17685)	44796..45497	-	702	WP_219191770.1	GAD-like domain-containing protein	-
KXJ74_RS17690 (KXJ74_17690)	46079..46768	+	690	WP_219191771.1	aspartate/glutamate racemase family protein	-
KXJ74_RS17695 (KXJ74_17695)	46958..47890	+	933	WP_219191772.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..71994	71994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11289.69 Da
• Isoelectric Point: 5.6113

>T209896 WP_107010192.1 NZ_CP079749:c42960-42673 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKNQKKHDISFETASLIFEDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T209896 NZ_CP104330:c2370174-2369998 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12195.76 Da
• Isoelectric Point: 9.5093

>AT209896 WP_107010193.1 NZ_CP079749:c42686-42372 [Acinetobacter johnsonii]
MSMVRYTREELNENFSDKQDAEIKRLLAKGTVSDNQLDLSDIPEITDWSNAVRHGQFYRPVKQQTSVRLDADVLAWLKSQ
GKGYQTRMNKILREAMLNERKNHQ

Nucleotide

• Length: 315 bp

>AT209896 NZ_CP104330:c2369952-2369536 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA