Detailed information of TA system TA209895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 35690..36281
• Replicon: plasmid pMRA
• Accession: NZ_CP079749
• Organism: Acinetobacter johnsonii strain MR1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: KXJ74_RS17625
• Protein ID: WP_219191785.1
• Coordinates: 35690..35977 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N8UJC9
• Locus tag: KXJ74_RS17630
• Protein ID: WP_004759458.1
• Coordinates: 35964..36281 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ74_RS17585 (KXJ74_17585)	30959..31231	+	273	WP_219191783.1	NadS family protein	-
KXJ74_RS17590 (KXJ74_17590)	31312..31818	-	507	WP_005254456.1	hypothetical protein	-
KXJ74_RS18445	31837..32103	-	267	WP_005254455.1	hypothetical protein	-
KXJ74_RS17595 (KXJ74_17595)	32104..32535	-	432	WP_005254452.1	hypothetical protein	-
KXJ74_RS17600 (KXJ74_17600)	32551..33480	-	930	WP_219191784.1	hypothetical protein	-
KXJ74_RS17605 (KXJ74_17605)	33702..33947	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KXJ74_RS17610 (KXJ74_17610)	33937..34230	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KXJ74_RS17615 (KXJ74_17615)	34323..34625	+	303	WP_136144098.1	hypothetical protein	-
KXJ74_RS17620 (KXJ74_17620)	34771..35376	-	606	WP_087696031.1	alpha/beta hydrolase	-
KXJ74_RS17625 (KXJ74_17625)	35690..35977	+	288	WP_219191785.1	BrnT family toxin	Toxin
KXJ74_RS17630 (KXJ74_17630)	35964..36281	+	318	WP_004759458.1	BrnA antitoxin family protein	Antitoxin
KXJ74_RS17640 (KXJ74_17640)	36473..37429	+	957	WP_219191767.1	hypothetical protein	-
KXJ74_RS17645 (KXJ74_17645)	37594..38964	+	1371	WP_219191768.1	SIR2 family protein	-
KXJ74_RS17650 (KXJ74_17650)	39379..39780	+	402	WP_219191769.1	hypothetical protein	-
KXJ74_RS17655 (KXJ74_17655)	39777..40295	+	519	WP_228738031.1	hypothetical protein	-
KXJ74_RS17660 (KXJ74_17660)	40636..41247	+	612	WP_077169897.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..71994	71994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11321.69 Da
• Isoelectric Point: 5.2216

>T209895 WP_219191785.1 NZ_CP079749:35690-35977 [Acinetobacter johnsonii]
MEQYFEWDETKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T209895 NZ_CP104330:2287284-2288606 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12367.08 Da
• Isoelectric Point: 9.7969

>AT209895 WP_004759458.1 NZ_CP079749:35964-36281 [Acinetobacter johnsonii]
MSMVRYSRKELNKKFSDKQDAEIERLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHKN

Nucleotide

• Length: 318 bp

>AT209895 NZ_CP104330:2287018-2287284 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: N8UJC9