Detailed information of TA system TA209894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 33702..34230
• Replicon: plasmid pMRA
• Accession: NZ_CP079749
• Organism: Acinetobacter johnsonii strain MR1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9N871
• Locus tag: KXJ74_RS17610
• Protein ID: WP_000221358.1
• Coordinates: 33937..34230 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: KXJ74_RS17605
• Protein ID: WP_000246756.1
• Coordinates: 33702..33947 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ74_RS18440	29319..30341	+	1023	WP_228738035.1	hypothetical protein	-
KXJ74_RS17580 (KXJ74_17580)	30646..30966	+	321	WP_219191782.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KXJ74_RS17585 (KXJ74_17585)	30959..31231	+	273	WP_219191783.1	NadS family protein	-
KXJ74_RS17590 (KXJ74_17590)	31312..31818	-	507	WP_005254456.1	hypothetical protein	-
KXJ74_RS18445	31837..32103	-	267	WP_005254455.1	hypothetical protein	-
KXJ74_RS17595 (KXJ74_17595)	32104..32535	-	432	WP_005254452.1	hypothetical protein	-
KXJ74_RS17600 (KXJ74_17600)	32551..33480	-	930	WP_219191784.1	hypothetical protein	-
KXJ74_RS17605 (KXJ74_17605)	33702..33947	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KXJ74_RS17610 (KXJ74_17610)	33937..34230	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KXJ74_RS17615 (KXJ74_17615)	34323..34625	+	303	WP_136144098.1	hypothetical protein	-
KXJ74_RS17620 (KXJ74_17620)	34771..35376	-	606	WP_087696031.1	alpha/beta hydrolase	-
KXJ74_RS17625 (KXJ74_17625)	35690..35977	+	288	WP_219191785.1	BrnT family toxin	-
KXJ74_RS17630 (KXJ74_17630)	35964..36281	+	318	WP_004759458.1	BrnA antitoxin family protein	-
KXJ74_RS17640 (KXJ74_17640)	36473..37429	+	957	WP_219191767.1	hypothetical protein	-
KXJ74_RS17645 (KXJ74_17645)	37594..38964	+	1371	WP_219191768.1	SIR2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..71994	71994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11292.52 Da
• Isoelectric Point: 10.4753

>T209894 WP_000221358.1 NZ_CP079749:33937-34230 [Acinetobacter johnsonii]
MTYKLLRHKDFTAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T209894 NZ_CP104330:2233827-2234114 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT209894 WP_000246756.1 NZ_CP079749:33702-33947 [Acinetobacter johnsonii]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT209894 NZ_CP104330:2233588-2233827 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A6A1RKP4

Antitoxin

• AlphaFold DB: N9B5Q7