Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4071202..4071699 Replicon chromosome
Accession NZ_CP079747
Organism Escherichia coli strain DL21

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KXJ73_RS19870 Protein ID WP_001173343.1
Coordinates 4071202..4071375 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KXJ73_RS19875 Protein ID WP_000398619.1
Coordinates 4071403..4071699 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXJ73_RS19860 (4067872) 4067872..4069329 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
KXJ73_RS19865 (4069566) 4069566..4071083 + 1518 WP_001295090.1 lysine--tRNA ligase -
KXJ73_RS19870 (4071202) 4071202..4071375 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KXJ73_RS19875 (4071403) 4071403..4071699 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KXJ73_RS19880 (4071926) 4071926..4072198 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KXJ73_RS19885 (4072210) 4072210..4072440 - 231 WP_001070602.1 4Fe-4S mono-cluster protein YjdI -
KXJ73_RS19890 (4072621) 4072621..4074257 + 1637 Protein_3897 sensor histidine kinase -
KXJ73_RS19895 (4074254) 4074254..4074973 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T209891 WP_001173343.1 NZ_CP079747:c4071375-4071202 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T209891 NZ_CP104330:1790771-1791025 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT209891 WP_000398619.1 NZ_CP079747:c4071699-4071403 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT209891 NZ_CP104330:1790523-1790774 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References