209887

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3358110..3358947 Replicon chromosome
Accession NZ_CP079747
Organism Escherichia coli strain DL21

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag KXJ73_RS16440 Protein ID WP_000227786.1
Coordinates 3358405..3358947 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag KXJ73_RS16435 Protein ID WP_001297137.1
Coordinates 3358110..3358421 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXJ73_RS16410 (3353130) 3353130..3354077 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
KXJ73_RS16415 (3354099) 3354099..3356090 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KXJ73_RS16420 (3356080) 3356080..3356694 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
KXJ73_RS16425 (3356694) 3356694..3357023 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
KXJ73_RS16430 (3357035) 3357035..3357925 + 891 WP_000971336.1 heme o synthase -
KXJ73_RS16435 (3358110) 3358110..3358421 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
KXJ73_RS16440 (3358405) 3358405..3358947 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
KXJ73_RS16445 (3359003) 3359003..3359938 - 936 WP_001297127.1 tetratricopeptide repeat protein -
KXJ73_RS16450 (3360355) 3360355..3361719 + 1365 WP_001000978.1 MFS transporter -
KXJ73_RS16455 (3361847) 3361847..3362338 - 492 WP_001138904.1 nucleotide binding protein YajQ -
KXJ73_RS16460 (3362506) 3362506..3363417 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T209887 WP_000227786.1 NZ_CP079747:3358405-3358947 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T209887 NZ_CP104330:974677-975012 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT209887 WP_001297137.1 NZ_CP079747:3358110-3358421 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT209887 NZ_CP104330:974429-974677 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References