Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3322670..3323288 Replicon chromosome
Accession NZ_CP079747
Organism Escherichia coli strain DL21

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KXJ73_RS16265 Protein ID WP_001291435.1
Coordinates 3323070..3323288 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KXJ73_RS16260 Protein ID WP_000344800.1
Coordinates 3322670..3323044 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXJ73_RS16250 (3317759) 3317759..3318952 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KXJ73_RS16255 (3318975) 3318975..3322124 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KXJ73_RS16260 (3322670) 3322670..3323044 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KXJ73_RS16265 (3323070) 3323070..3323288 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KXJ73_RS16270 (3323460) 3323460..3324011 + 552 WP_000102564.1 maltose O-acetyltransferase -
KXJ73_RS16275 (3324127) 3324127..3324597 + 471 WP_000136192.1 YlaC family protein -
KXJ73_RS16280 (3324761) 3324761..3326311 + 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KXJ73_RS16285 (3326353) 3326353..3326706 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KXJ73_RS16295 (3327085) 3327085..3327396 + 312 WP_000409911.1 MGMT family protein -
KXJ73_RS16300 (3327427) 3327427..3327999 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209886 WP_001291435.1 NZ_CP079747:3323070-3323288 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T209886 NZ_CP104330:844945-845352 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT209886 WP_000344800.1 NZ_CP079747:3322670-3323044 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT209886 NZ_CP104330:844698-844964 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References