Detailed information of TA system TA209885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2308463..2309101
• Replicon: chromosome
• Accession: NZ_CP079747
• Organism: Escherichia coli strain DL21

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KXJ73_RS11110
• Protein ID: WP_000813794.1
• Coordinates: 2308925..2309101 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KXJ73_RS11105
• Protein ID: WP_001270286.1
• Coordinates: 2308463..2308879 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ73_RS11085 (2303615)	2303615..2304556	-	942	WP_001251313.1	ABC transporter permease	-
KXJ73_RS11090 (2304557)	2304557..2305570	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KXJ73_RS11095 (2305588)	2305588..2306733	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KXJ73_RS11100 (2306978)	2306978..2308384	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KXJ73_RS11105 (2308463)	2308463..2308879	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KXJ73_RS11110 (2308925)	2308925..2309101	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KXJ73_RS11115 (2309323)	2309323..2309553	+	231	WP_000494244.1	YncJ family protein	-
KXJ73_RS11120 (2309645)	2309645..2311606	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KXJ73_RS11125 (2311679)	2311679..2312215	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KXJ73_RS11130 (2312268)	2312268..2313482	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T209885 WP_000813794.1 NZ_CP079747:c2309101-2308925 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T209885 NZ_CP104330:717224-717520 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT209885 WP_001270286.1 NZ_CP079747:c2308879-2308463 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT209885 NZ_CP104330:717522-717917 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 6HPB