Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 227947..228481 Replicon plasmid pB1A
Accession NZ_CP079746
Organism Comamonas aquatica strain B1

Toxin (Protein)


Gene name relE Uniprot ID A0A4D4JP15
Locus tag KXJ72_RS18040 Protein ID WP_013521176.1
Coordinates 228200..228481 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A4D4JMS0
Locus tag KXJ72_RS18035 Protein ID WP_013521177.1
Coordinates 227947..228213 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXJ72_RS18015 (KXJ72_18020) 223641..224666 + 1026 WP_219165004.1 hypothetical protein -
KXJ72_RS18020 (KXJ72_18025) 224735..226897 + 2163 WP_219165005.1 tyrosine-type recombinase/integrase -
KXJ72_RS18025 (KXJ72_18030) 226942..227679 + 738 WP_219165007.1 hypothetical protein -
KXJ72_RS18035 (KXJ72_18040) 227947..228213 + 267 WP_013521177.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KXJ72_RS18040 (KXJ72_18045) 228200..228481 + 282 WP_013521176.1 type II toxin-antitoxin system YafQ family toxin Toxin
KXJ72_RS18045 (KXJ72_18050) 228592..229489 + 898 Protein_237 Tn3 family transposase -
KXJ72_RS18050 (KXJ72_18055) 229784..230671 - 888 WP_066553272.1 LysR substrate-binding domain-containing protein -
KXJ72_RS18055 (KXJ72_18060) 230861..231406 + 546 Protein_239 ATP-binding cassette domain-containing protein -
KXJ72_RS18060 (KXJ72_18065) 231407..232069 + 663 WP_013521175.1 peroxiredoxin-like family protein -
KXJ72_RS18065 (KXJ72_18070) 232066..233055 + 990 WP_013521174.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaIMP-8 / msr(E) / mph(E) / aph(3'')-Ib / aph(6)-Id / sul1 - 1..295765 295765
- flank IS/Tn - - 228689..229489 800


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(2-93)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10611.02 Da        Isoelectric Point: 8.9597

>T209871 WP_013521176.1 NZ_CP079746:228200-228481 [Comamonas aquatica]
MRTIDRASAFKRDYKREAKGQHRATLDSDLKPVLVALATDQPLDAKFRDHDLSGDWAGYRECHVKPDLLLIYRKSDADTL
RLARLGSHSELFG

Download         Length: 282 bp

>T209871 NZ_CP104328:c3522835-3522494 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9654.00 Da        Isoelectric Point: 5.1392

>AT209871 WP_013521177.1 NZ_CP079746:227947-228213 [Comamonas aquatica]
MSTADTYVRARIDTSTKERAADALEAMGLSISDAIRLLMLRIADERRMPFDVKAPNATTRKAIAELEAGKGKRFASVDDL
MADLHADD

Download         Length: 267 bp

>AT209871 NZ_CP104328:c3523173-3522856 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D4JP15

Antitoxin

Source ID Structure
AlphaFold DB A0A4D4JMS0

References