Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 199817..200447 Replicon chromosome
Accession NZ_CP079737
Organism Paraclostridium bifermentans strain DSM 14991

Toxin (Protein)


Gene name mazF Uniprot ID T4VJE6
Locus tag KXZ80_RS01255 Protein ID WP_021430410.1
Coordinates 200082..200447 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0M3DH37
Locus tag KXZ80_RS01250 Protein ID WP_035118413.1
Coordinates 199817..200092 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXZ80_RS01220 (KXZ80_01220) 195260..196654 + 1395 WP_021430421.1 F0F1 ATP synthase subunit beta -
KXZ80_RS01225 (KXZ80_01225) 196657..196917 + 261 WP_021430426.1 ATP synthase F1 subunit epsilon -
KXZ80_RS01230 (KXZ80_01230) 197137..197517 + 381 WP_021434187.1 holo-ACP synthase -
KXZ80_RS01235 (KXZ80_01235) 197522..197983 + 462 WP_021434186.1 CBS domain-containing protein -
KXZ80_RS01240 (KXZ80_01240) 198006..198596 + 591 WP_021434185.1 germination lipoprotein GerS -
KXZ80_RS01245 (KXZ80_01245) 198611..199774 + 1164 WP_021434184.1 alanine racemase -
KXZ80_RS01250 (KXZ80_01250) 199817..200092 + 276 WP_035118413.1 hypothetical protein Antitoxin
KXZ80_RS01255 (KXZ80_01255) 200082..200447 + 366 WP_021430410.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KXZ80_RS01260 (KXZ80_01260) 200612..201439 + 828 WP_021434183.1 transketolase -
KXZ80_RS01265 (KXZ80_01265) 201432..202367 + 936 WP_021434182.1 transketolase family protein -
KXZ80_RS01270 (KXZ80_01270) 202486..202911 + 426 WP_025161277.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-118)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13480.63 Da        Isoelectric Point: 8.0154

>T209864 WP_021430410.1 NZ_CP079737:200082-200447 [Paraclostridium bifermentans]
VSSNNLDIKRGHLFYADLSPVVGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHIEISSNEYGLNKDSVI
LLEQIRTIDKKRLREKIGYLDECMMEKVNNSLLISLGLFQI

Download         Length: 366 bp

>T209864 NZ_CP104328:2234130-2234417 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10338.72 Da        Isoelectric Point: 4.3885

>AT209864 WP_035118413.1 NZ_CP079737:199817-200092 [Paraclostridium bifermentans]
MSKEKIVFTLPDSLISEVDHIVQIENSNREDFAKAAFQFYITQKKKFDLKESMIKGYKEMGQINLTLAELGMTNEVSSDD
NLEGTIAQGEF

Download         Length: 276 bp

>AT209864 NZ_CP104328:2233891-2234130 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T4VJE6

Antitoxin

Source ID Structure
AlphaFold DB A0A0M3DH37

References