Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4091238..4091735 Replicon chromosome
Accession NZ_CP079736
Organism Escherichia coli strain GB2005-delet-a-f

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KYX13_RS20055 Protein ID WP_001173343.1
Coordinates 4091238..4091411 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KYX13_RS20060 Protein ID WP_000398619.1
Coordinates 4091439..4091735 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX13_RS20045 (4087908) 4087908..4089365 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KYX13_RS20050 (4089602) 4089602..4091119 + 1518 WP_001295074.1 lysine--tRNA ligase -
KYX13_RS20055 (4091238) 4091238..4091411 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KYX13_RS20060 (4091439) 4091439..4091735 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KYX13_RS20065 (4091962) 4091962..4092234 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KYX13_RS20070 (4092246) 4092246..4092476 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KYX13_RS20075 (4092657) 4092657..4094288 + 1632 WP_001216477.1 sensor histidine kinase -
KYX13_RS20080 (4094285) 4094285..4095004 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T209863 WP_001173343.1 NZ_CP079736:c4091411-4091238 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T209863 NZ_CP104328:1956556-1956659 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT209863 WP_000398619.1 NZ_CP079736:c4091735-4091439 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT209863 NZ_CP104328:c1956697-1956426 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References