Detailed information of TA system TA020985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3431722..3432374
• Replicon: chromosome
• Accession: NC_005125
• Organism: Gloeobacter violaceus PCC 7421

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GLL_RS16645
• Protein ID: WP_011143215.1
• Coordinates: 3431997..3432374 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q7NGE7
• Locus tag: GLL_RS16640
• Protein ID: WP_011143214.1
• Coordinates: 3431722..3432000 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLL_RS16615	3427144..3428031	+	888	WP_011143210.1	GNAT family N-acetyltransferase	-
GLL_RS16620	3428025..3428291	-	267	WP_164929222.1	hypothetical protein	-
GLL_RS16625	3429519..3429863	+	345	WP_011143212.1	helix-turn-helix domain-containing protein	-
GLL_RS16630	3429864..3430181	+	318	WP_011143213.1	transposase	-
GLL_RS16635	3430461..3431090	-	630	WP_164929223.1	hypothetical protein	-
GLL_RS16640	3431722..3432000	+	279	WP_011143214.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GLL_RS16645	3431997..3432374	+	378	WP_011143215.1	type II toxin-antitoxin system VapC family toxin	Toxin
GLL_RS16650	3432641..3433075	-	435	WP_164929224.1	ester cyclase	-
GLL_RS16655	3433179..3434066	-	888	WP_011143217.1	AraC family transcriptional regulator	-
GLL_RS16660	3434407..3434604	+	198	WP_164929225.1	hypothetical protein	-
GLL_RS16665	3434680..3435810	+	1131	WP_164929226.1	UDP-N-acetylglucosamine--LPS N-acetylglucosamine transferase	-
GLL_RS16670	3435813..3437357	+	1545	WP_011143220.1	glycoside hydrolase family 1 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-118)

Antitoxin

• Antitoxin VapB-like (4-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13378.36 Da
• Isoelectric Point: 4.4002

>T20985 WP_011143215.1 NC_005125:3431997-3432374 [Gloeobacter violaceus PCC 7421]
MIAIDTSAIVAILLGEPEAERFARAIEQDEEPLLSAASLVELFLAMKHKQGSASVPLIEQFLATGGISVCSVTPEQARRA
QEAAYRFGVLNFGDVFSYALAKERGIGLLFKGTDFAVTDLTAAPF

Nucleotide

• Length: 378 bp

>T20985 NC_005125:3431997-3432374 [Gloeobacter violaceus PCC 7421]
ATGATCGCGATCGATACTTCCGCCATCGTCGCCATTCTCCTTGGTGAGCCCGAAGCGGAGCGCTTCGCACGCGCCATCGA
GCAGGACGAGGAACCGCTGCTTTCAGCGGCTTCCCTGGTTGAGCTTTTCCTCGCGATGAAGCACAAGCAGGGGTCGGCGT
CCGTGCCCTTGATCGAGCAGTTCCTGGCCACGGGCGGGATCAGCGTCTGTTCTGTCACCCCCGAGCAAGCCCGCCGCGCT
CAGGAAGCCGCCTATCGCTTCGGCGTGCTGAATTTTGGCGATGTGTTTTCTTATGCGCTCGCCAAGGAGAGGGGCATTGG
TCTTCTGTTCAAAGGTACCGACTTCGCGGTGACCGATCTCACCGCAGCACCCTTCTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10376.86 Da
• Isoelectric Point: 8.5610

>AT20985 WP_011143214.1 NC_005125:3431722-3432000 [Gloeobacter violaceus PCC 7421]
MQQINIKSDKAYRMANELSRLTGESVTQAITTAIEERLEKIRAQTRSRREGIAERLLALGTRSVALPILDPRAPDEILYD
ENGLPKNPMADR

Nucleotide

• Length: 279 bp

>AT20985 NC_005125:3431722-3432000 [Gloeobacter violaceus PCC 7421]
ATGCAGCAAATCAACATTAAAAGCGACAAAGCCTATCGAATGGCGAACGAACTGAGCCGCTTGACTGGGGAAAGCGTGAC
TCAGGCGATCACCACAGCCATTGAAGAGCGGCTCGAAAAGATTCGTGCCCAGACGCGCAGCCGCCGCGAGGGTATCGCCG
AACGACTGCTTGCCCTTGGCACAAGGAGCGTGGCTCTTCCCATCCTTGACCCGCGCGCGCCCGACGAGATCCTCTACGAC
GAGAACGGATTGCCGAAAAACCCGATGGCGGATCGATGA

Structures

Antitoxin

• AlphaFold DB: Q7NGE7