Detailed information of TA system TA209831

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3616812..3617491
• Replicon: chromosome
• Accession: NZ_CP079735
• Organism: Escherichia coli strain GB2005

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: KYX12_RS17675
• Protein ID: WP_000854672.1
• Coordinates: 3617150..3617491 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: KYX12_RS17670
• Protein ID: WP_000070395.1
• Coordinates: 3616812..3617129 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX12_RS17620 (3611859)	3611859..3612722	+	864	WP_001065553.1	GTPase family protein	-
KYX12_RS17625 (3612814)	3612814..3613635	+	822	WP_000197389.1	DUF932 domain-containing protein	-
KYX12_RS17630 (3613852)	3613852..3614043	+	192	Protein_3460	DeoR family transcriptional regulator	-
KYX12_RS17635 (3614090)	3614090..3614266	+	177	WP_001285112.1	hypothetical protein	-
KYX12_RS17640 (3614266)	3614266..3614709	+	444	WP_000824223.1	lipoprotein YafY	-
KYX12_RS17645 (3614732)	3614732..3615199	+	468	WP_001547765.1	protein YkfB	-
KYX12_RS17650 (3615276)	3615276..3615515	+	240	WP_000194654.1	DUF905 family protein	-
KYX12_RS17655 (3615613)	3615613..3616071	+	459	WP_000211838.1	antirestriction protein	-
KYX12_RS17660 (3616087)	3616087..3616563	+	477	WP_000811693.1	RadC family protein	-
KYX12_RS17665 (3616572)	3616572..3616793	+	222	WP_000691994.1	DUF987 domain-containing protein	-
KYX12_RS17670 (3616812)	3616812..3617129	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
KYX12_RS17675 (3617150)	3617150..3617491	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
KYX12_RS17685 (3618063)	3618063..3619316	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
KYX12_RS17690 (3619328)	3619328..3620431	-	1104	WP_001285288.1	glutamate 5-kinase	-
KYX12_RS17695 (3620719)	3620719..3621774	+	1056	WP_000749863.1	phosphoporin PhoE	-
KYX12_RS17700 (3621813)	3621813..3622214	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T209831 WP_000854672.1 NZ_CP079735:3617150-3617491 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T209831 NZ_CP104326:1796882-1797136 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT209831 WP_000070395.1 NZ_CP079735:3616812-3617129 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT209831 NZ_CP104326:1796634-1796885 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684