Detailed information of TA system TA209830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3433685..3434303
• Replicon: chromosome
• Accession: NZ_CP079735
• Organism: Escherichia coli strain GB2005

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: KYX12_RS16715
• Protein ID: WP_001290581.1
• Coordinates: 3434085..3434303 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: KYX12_RS16710
• Protein ID: WP_000344800.1
• Coordinates: 3433685..3434059 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX12_RS16700 (3428774)	3428774..3429967	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
KYX12_RS16705 (3429990)	3429990..3433139	+	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
KYX12_RS16710 (3433685)	3433685..3434059	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
KYX12_RS16715 (3434085)	3434085..3434303	+	219	WP_001290581.1	HHA domain-containing protein	Toxin
KYX12_RS16720 (3434475)	3434475..3435026	+	552	WP_000102564.1	maltose O-acetyltransferase	-
KYX12_RS16725 (3435142)	3435142..3435612	+	471	WP_000136192.1	YlaC family protein	-
KYX12_RS16730 (3435776)	3435776..3437326	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
KYX12_RS16735 (3437368)	3437368..3437721	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
KYX12_RS16745 (3438100)	3438100..3438411	+	312	WP_000409911.1	MGMT family protein	-
KYX12_RS16750 (3438442)	3438442..3439014	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T209830 WP_001290581.1 NZ_CP079735:3434085-3434303 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T209830 NZ_CP104326:1796634-1796885 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT209830 WP_000344800.1 NZ_CP079735:3433685-3434059 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT209830 NZ_CP104326:1796882-1797136 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5