Detailed information of TA system TA209818

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1802627..1803458
• Replicon: chromosome
• Accession: NZ_CP079735
• Organism: Escherichia coli strain GB2005

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: KYX12_RS08600
• Protein ID: WP_000854814.1
• Coordinates: 1802627..1803001 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: KYX12_RS08605
• Protein ID: WP_001285584.1
• Coordinates: 1803090..1803458 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX12_RS08560 (1798023)	1798023..1799189	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
KYX12_RS08565 (1799308)	1799308..1799781	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
KYX12_RS08570 (1799979)	1799979..1801037	+	1059	WP_001200891.1	FUSC family protein	-
KYX12_RS08575 (1801209)	1801209..1801538	+	330	WP_000450409.1	DUF496 family protein	-
KYX12_RS08580 (1801639)	1801639..1801773	-	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
KYX12_RS08585 (1801893)	1801893..1802021	+	129	Protein_1681	transposase domain-containing protein	-
KYX12_RS08590 (1802310)	1802310..1802390	-	81	Protein_1682	hypothetical protein	-
KYX12_RS08595 (1802436)	1802436..1802630	-	195	WP_000988600.1	DUF5983 family protein	-
KYX12_RS08600 (1802627)	1802627..1803001	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KYX12_RS08605 (1803090)	1803090..1803458	-	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KYX12_RS08610 (1803532)	1803532..1803753	-	222	WP_000692323.1	DUF987 domain-containing protein	-
KYX12_RS08615 (1803816)	1803816..1804262	-	447	WP_000187523.1	RadC family protein	-
KYX12_RS08620 (1804259)	1804259..1805791	-	1533	WP_001350525.1	protein YeeR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T209818 WP_000854814.1 NZ_CP079735:c1803001-1802627 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T209818 NZ_CP104317:c6166228-6165881 [Streptomyces angustmyceticus]
ATGAGGCGTGGTGACATCTACATGGTCGACCTGGAGCCGACGCGCGGCAGTGAGGCGAACAAGATCCGCCCGGCCCTGAT
CGTGTCCCACAACGGCGCCAATCAGTCGGTCGAGGCTCACCGGCGTGGGGTGGTCACGGTCGTTCCCCTGACGTCGAACA
CCTCGCGGGTGCTCTCCTTCCAGGTCCTCCTCGAGGCCGAGGAGTGCCGTCTGCCCAAGGACTCCAAAGCGCAGTGCGAG
CAACTGCGCGCCGTCGCGCCGGAACGCGTGCTCCACAAGGTCGGCGTGGTGCCGCGCCGGCGCATGGCCGAGGTCGATGC
CGCGCTGCGCCGCCATCTGGCGCTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT209818 WP_001285584.1 NZ_CP079735:c1803458-1803090 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT209818 NZ_CP104317:c6166445-6166215 [Streptomyces angustmyceticus]
ATGAAGATCAGTGTGAGCCTGCCGCAGGAGGACGTGGCCTTCGTCGACGAATACGCGGCGAGAACGGCGGGGGAATCGCG
GTCCGCCGTGATACACGCTGCCATCGAGCTGCTGCGTGCCGCGCAGCTCGAAGCCGAGTACACCGAAGCGTTCGCGGAGT
GGGACACCAGCGAGGACGCGGCACTGTGGGACCGCACGAGCCGGGACGGACTTGCCGATGAGGCGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6