Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 851828..852482 Replicon chromosome
Accession NZ_CP079735
Organism Escherichia coli strain GB2005

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KYX12_RS04145 Protein ID WP_000244777.1
Coordinates 852075..852482 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KYX12_RS04140 Protein ID WP_000354046.1
Coordinates 851828..852094 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX12_RS04115 (846997) 846997..847740 + 744 WP_000951964.1 SDR family oxidoreductase -
KYX12_RS04120 (847797) 847797..849230 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KYX12_RS04125 (849275) 849275..849586 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KYX12_RS04130 (849750) 849750..850409 + 660 WP_000250274.1 hemolysin III family protein -
KYX12_RS04135 (850605) 850605..851585 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KYX12_RS04140 (851828) 851828..852094 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KYX12_RS04145 (852075) 852075..852482 + 408 WP_000244777.1 protein YgfX Toxin
KYX12_RS04150 (852522) 852522..853043 - 522 WP_001055874.1 flavodoxin FldB -
KYX12_RS04155 (853155) 853155..854051 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KYX12_RS04160 (854076) 854076..854786 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KYX12_RS04165 (854792) 854792..856525 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T209813 WP_000244777.1 NZ_CP079735:852075-852482 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T209813 NZ_CP104315:101933-102082 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT209813 WP_000354046.1 NZ_CP079735:851828-852094 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT209813 NZ_CP104315:c101888-101829 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References