Detailed information of TA system TA209809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 58719..59244
• Replicon: plasmid pKP3295-2
• Accession: NZ_CP079727
• Organism: Klebsiella pneumoniae strain KP3295

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: KXI30_RS26840
• Protein ID: WP_013023785.1
• Coordinates: 58939..59244 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1D8K3R4
• Locus tag: KXI30_RS26835
• Protein ID: WP_006788213.1
• Coordinates: 58719..58937 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXI30_RS28375	54239..54775	+	537	WP_223825639.1	hypothetical protein	-
KXI30_RS28380	54793..55116	+	324	WP_085842301.1	hypothetical protein	-
KXI30_RS26810 (KXI30_26800)	55157..55573	-	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	-
KXI30_RS26815 (KXI30_26805)	55570..55800	-	231	WP_016338373.1	type II toxin-antitoxin system VapB family antitoxin	-
KXI30_RS26820 (KXI30_26810)	56018..56932	-	915	WP_126834912.1	Abi family protein	-
KXI30_RS26825 (KXI30_26815)	57225..58178	-	954	WP_109549184.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KXI30_RS26830 (KXI30_26820)	58286..58549	+	264	Protein_68	hypothetical protein	-
KXI30_RS26835 (KXI30_26825)	58719..58937	+	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KXI30_RS26840 (KXI30_26830)	58939..59244	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
KXI30_RS26845 (KXI30_26835)	59413..59808	+	396	WP_017899885.1	hypothetical protein	-
KXI30_RS26850 (KXI30_26840)	59835..60149	+	315	WP_053389906.1	hypothetical protein	-
KXI30_RS26855 (KXI30_26845)	60160..61176	+	1017	WP_017899884.1	hypothetical protein	-
KXI30_RS26860 (KXI30_26850)	61374..62168	+	795	WP_004197635.1	site-specific integrase	-
KXI30_RS26865 (KXI30_26855)	62616..62918	-	303	WP_071571079.1	hypothetical protein	-
KXI30_RS26870 (KXI30_26860)	62915..63541	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB1 / dfrA14 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..133750	133750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T209809 WP_013023785.1 NZ_CP079727:58939-59244 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T209809 NZ_CP104312:c4843137-4842853 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7405

>AT209809 WP_006788213.1 NZ_CP079727:58719-58937 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT209809 NZ_CP104312:c4843369-4843127 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A1D8K3R4