Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 55157..55800 Replicon plasmid pKP3295-2
Accession NZ_CP079727
Organism Klebsiella pneumoniae strain KP3295

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag KXI30_RS26810 Protein ID WP_032425563.1
Coordinates 55157..55573 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag KXI30_RS26815 Protein ID WP_016338373.1
Coordinates 55570..55800 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXI30_RS26795 (KXI30_26785) 50205..52313 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
KXI30_RS26800 (KXI30_26790) 52303..53580 - 1278 WP_016338369.1 HlyD family secretion protein -
KXI30_RS28375 54239..54775 + 537 WP_223825639.1 hypothetical protein -
KXI30_RS28380 54793..55116 + 324 WP_085842301.1 hypothetical protein -
KXI30_RS26810 (KXI30_26800) 55157..55573 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
KXI30_RS26815 (KXI30_26805) 55570..55800 - 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KXI30_RS26820 (KXI30_26810) 56018..56932 - 915 WP_126834912.1 Abi family protein -
KXI30_RS26825 (KXI30_26815) 57225..58178 - 954 WP_109549184.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
KXI30_RS26830 (KXI30_26820) 58286..58549 + 264 Protein_68 hypothetical protein -
KXI30_RS26835 (KXI30_26825) 58719..58937 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
KXI30_RS26840 (KXI30_26830) 58939..59244 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
KXI30_RS26845 (KXI30_26835) 59413..59808 + 396 WP_017899885.1 hypothetical protein -
KXI30_RS26850 (KXI30_26840) 59835..60149 + 315 WP_053389906.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB1 / dfrA14 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..133750 133750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T209808 WP_032425563.1 NZ_CP079727:c55573-55157 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T209808 NZ_CP104312:c4738787-4738254 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT209808 WP_016338373.1 NZ_CP079727:c55800-55570 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT209808 NZ_CP104312:c4739064-4738798 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References