Detailed information of TA system TA209806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 104029..104699
• Replicon: plasmid pKP3295-1
• Accession: NZ_CP079726
• Organism: Klebsiella pneumoniae strain KP3295

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KXI30_RS26105
• Protein ID: WP_004213072.1
• Coordinates: 104029..104472 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KXI30_RS26110
• Protein ID: WP_004213073.1
• Coordinates: 104469..104699 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXI30_RS26070 (KXI30_26060)	99438..99713	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
KXI30_RS26075 (KXI30_26065)	99776..100267	+	492	WP_011251327.1	DM13 domain-containing protein	-
KXI30_RS26080 (KXI30_26070)	100316..101236	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
KXI30_RS26085 (KXI30_26075)	101327..101730	+	404	Protein_105	GAF domain-containing protein	-
KXI30_RS26090 (KXI30_26080)	102248..102886	-	639	WP_032488580.1	mucoid phenotype regulator RmpA2	-
KXI30_RS26095 (KXI30_26085)	103302..103606	+	305	Protein_107	transposase	-
KXI30_RS28340	103629..103880	-	252	WP_186987481.1	hypothetical protein	-
KXI30_RS26105 (KXI30_26095)	104029..104472	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KXI30_RS26110 (KXI30_26100)	104469..104699	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KXI30_RS26115 (KXI30_26105)	105307..106440	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
KXI30_RS26120 (KXI30_26110)	106456..106749	+	294	WP_004213076.1	hypothetical protein	-
KXI30_RS26125 (KXI30_26115)	106739..106945	-	207	WP_004213077.1	hypothetical protein	-
KXI30_RS26130 (KXI30_26120)	107297..107587	+	291	WP_004213078.1	hypothetical protein	-
KXI30_RS26135 (KXI30_26125)	107577..108476	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA	1..177848	177848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T209806 WP_004213072.1 NZ_CP079726:c104472-104029 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T209806 NZ_CP104312:c3304247-3304071 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT209806 WP_004213073.1 NZ_CP079726:c104699-104469 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT209806 NZ_CP104312:c3304023-3303616 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCTTGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGTACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620