Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5251075..5251700 Replicon chromosome
Accession NZ_CP079725
Organism Klebsiella pneumoniae strain KP3295

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KXI30_RS25205 Protein ID WP_002882817.1
Coordinates 5251075..5251458 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KXI30_RS25210 Protein ID WP_004150355.1
Coordinates 5251458..5251700 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXI30_RS25190 (5248441) 5248441..5249343 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KXI30_RS25195 (5249340) 5249340..5249975 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KXI30_RS25200 (5249972) 5249972..5250901 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KXI30_RS25205 (5251075) 5251075..5251458 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KXI30_RS25210 (5251458) 5251458..5251700 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KXI30_RS25215 (5251905) 5251905..5252822 + 918 WP_004181612.1 alpha/beta hydrolase -
KXI30_RS25220 (5252836) 5252836..5253777 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KXI30_RS25225 (5253822) 5253822..5254259 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KXI30_RS25230 (5254256) 5254256..5255116 - 861 WP_004146232.1 virulence factor BrkB family protein -
KXI30_RS25235 (5255110) 5255110..5255709 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209804 WP_002882817.1 NZ_CP079725:c5251458-5251075 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209804 NZ_CP104312:1980224-1980326 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209804 WP_004150355.1 NZ_CP079725:c5251700-5251458 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209804 NZ_CP104312:c1980333-1980188 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References