Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4687054..4687864 Replicon chromosome
Accession NZ_CP079725
Organism Klebsiella pneumoniae strain KP3295

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KXI30_RS22545 Protein ID WP_004178461.1
Coordinates 4687054..4687587 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KXI30_RS22550 Protein ID WP_002887278.1
Coordinates 4687598..4687864 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXI30_RS22540 (4685885) 4685885..4687006 + 1122 WP_002887282.1 cupin domain-containing protein -
KXI30_RS22545 (4687054) 4687054..4687587 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KXI30_RS22550 (4687598) 4687598..4687864 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KXI30_RS22555 (4687967) 4687967..4689400 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KXI30_RS22560 (4689390) 4689390..4690073 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KXI30_RS22565 (4690245) 4690245..4691630 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KXI30_RS22570 (4691648) 4691648..4691992 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(91-151)

Antitoxin

(2-86)


Sequences


Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209801 WP_004178461.1 NZ_CP079725:c4687587-4687054 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209801 NZ_CP104312:357717-358064 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209801 WP_002887278.1 NZ_CP079725:c4687864-4687598 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209801 NZ_CP104312:358064-358363 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A060VJ83


Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References