Detailed information of TA system TA209786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 894879..895515
• Replicon: chromosome
• Accession: NZ_CP079720
• Organism: Heyndrickxia oleronia strain FW

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0Q3WTD1
• Locus tag: KXF32_RS04680
• Protein ID: WP_055741345.1
• Coordinates: 894879..895229 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A8E2I5A7
• Locus tag: KXF32_RS04685
• Protein ID: WP_058005316.1
• Coordinates: 895234..895515 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXF32_RS04645	890086..890883	-	798	WP_071975415.1	RNA polymerase sigma factor SigB	-
KXF32_RS04650	890861..891335	-	475	Protein_885	anti-sigma B factor RsbW	-
KXF32_RS04655	891335..891664	-	330	WP_058005321.1	anti-sigma factor antagonist	-
KXF32_RS04660	891740..892747	-	1008	WP_058005320.1	PP2C family protein-serine/threonine phosphatase	-
KXF32_RS04665	892759..893160	-	402	WP_058005319.1	anti-sigma regulatory factor	-
KXF32_RS04670	893165..893527	-	363	WP_139254832.1	STAS domain-containing protein	-
KXF32_RS04675	893518..894348	-	831	WP_058005317.1	RsbT co-antagonist protein RsbRA	-
KXF32_RS04680	894879..895229	-	351	WP_055741345.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KXF32_RS04685	895234..895515	-	282	WP_058005316.1	hypothetical protein	Antitoxin
KXF32_RS04690	895685..896860	-	1176	WP_079991325.1	alanine racemase	-
KXF32_RS04695	897226..898242	-	1017	WP_058005315.1	outer membrane lipoprotein carrier protein LolA	-
KXF32_RS04700	898447..898800	-	354	WP_058005314.1	holo-ACP synthase	-
KXF32_RS04705	898878..899468	+	591	WP_058005313.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13020.06 Da
• Isoelectric Point: 4.8998

>T209786 WP_055741345.1 NZ_CP079720:c895229-894879 [Heyndrickxia oleronia]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMEKVDDALQISLGLIEF

Nucleotide

• Length: 351 bp

>T209786 NZ_CP104309:2676520-2676696 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10774.26 Da
• Isoelectric Point: 6.4777

>AT209786 WP_058005316.1 NZ_CP079720:c895515-895234 [Heyndrickxia oleronia]
VSESSATTEILIRLPVHLLSELDGFAEQENVNRNEFIYRATKMYLRERKKRQIRESMRRGYMEMAKINLAIASEAIQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT209786 NZ_CP104309:2676742-2677158 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0Q3WTD1