Detailed information of TA system TA209785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1276691..1277608
• Replicon: chromosome
• Accession: NZ_CP079719
• Organism: Bacillus velezensis strain LABIM44

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: KXY09_RS06605
• Protein ID: WP_007407256.1
• Coordinates: 1276862..1277608 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KXY09_RS06600
• Protein ID: WP_003154807.1
• Coordinates: 1276691..1276861 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXY09_RS06565 (1271924)	1271924..1273546	+	1623	WP_076423972.1	pyocin knob domain-containing protein	-
KXY09_RS06570 (1273559)	1273559..1273930	+	372	WP_136396299.1	XkdW family protein	-
KXY09_RS06575 (1273935)	1273935..1274132	+	198	WP_007610833.1	XkdX family protein	-
KXY09_RS06580 (1274189)	1274189..1274950	+	762	WP_015239693.1	hypothetical protein	-
KXY09_RS06585 (1275002)	1275002..1275265	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KXY09_RS06590 (1275279)	1275279..1275542	+	264	WP_003154813.1	phage holin	-
KXY09_RS06595 (1275556)	1275556..1276434	+	879	WP_043021264.1	N-acetylmuramoyl-L-alanine amidase	-
KXY09_RS20170 (1276469)	1276469..1276594	-	126	WP_023356844.1	hypothetical protein	-
KXY09_RS06600 (1276691)	1276691..1276861	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KXY09_RS06605 (1276862)	1276862..1277608	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KXY09_RS06610 (1277713)	1277713..1278711	-	999	WP_007407255.1	inorganic phosphate transporter	-
KXY09_RS06615 (1278724)	1278724..1279341	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KXY09_RS06620 (1279627)	1279627..1280943	-	1317	WP_007610842.1	amino acid permease	-
KXY09_RS06625 (1281268)	1281268..1282218	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1241709..1285875	44166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T209785 WP_007407256.1 NZ_CP079719:c1277608-1276862 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T209785 NZ_CP104309:c2574260-2574111 [Escherichia coli O157:H7]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT209785 WP_003154807.1 NZ_CP079719:c1276861-1276691 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT209785 NZ_CP104309:2573889-2574067 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14