Detailed information of TA system TA209772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2684922..2685451
• Replicon: chromosome
• Accession: NZ_CP079715
• Organism: Staphylococcus aureus strain V8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KX797_RS13570
• Protein ID: WP_000621175.1
• Coordinates: 2684922..2685284 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KX797_RS13575
• Protein ID: WP_000948331.1
• Coordinates: 2685281..2685451 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KX797_RS13550 (2681900)	2681900..2682670	-	771	WP_219098869.1	RNA polymerase sigma factor SigB	-
KX797_RS13555 (2682645)	2682645..2683124	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KX797_RS13560 (2683126)	2683126..2683452	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KX797_RS13565 (2683571)	2683571..2684572	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KX797_RS13570 (2684922)	2684922..2685284	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KX797_RS13575 (2685281)	2685281..2685451	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KX797_RS13580 (2685536)	2685536..2686684	-	1149	WP_001281145.1	alanine racemase	-
KX797_RS13585 (2686750)	2686750..2687109	-	360	WP_000581200.1	holo-ACP synthase	-
KX797_RS13590 (2687113)	2687113..2687604	-	492	WP_001205910.1	PH domain-containing protein	-
KX797_RS13595 (2687591)	2687591..2689174	-	1584	WP_219098870.1	PH domain-containing protein	-
KX797_RS13600 (2689167)	2689167..2689646	-	480	WP_001287088.1	hypothetical protein	-
KX797_RS13605 (2689855)	2689855..2690415	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T209772 WP_000621175.1 NZ_CP079715:c2685284-2684922 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T209772 NZ_CP104303:c1805580-1805278 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT209772 WP_000948331.1 NZ_CP079715:c2685451-2685281 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT209772 NZ_CP104303:c1805842-1805573 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1