Detailed information of TA system TA020977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 123..600
• Replicon: plasmid pAsa3
• Accession: NC_004924
• Organism: Aeromonas salmonicida subsp. salmonicida A449

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8T8CMR8
• Locus tag: ASA_RS23705
• Protein ID: WP_073531821.1
• Coordinates: 331..600 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: Q7X2E4
• Locus tag: ASA_RS00095
• Protein ID: WP_005321784.1
• Coordinates: 123..347 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASA_RS00095	123..347	+	225	WP_005321784.1	TraY domain-containing protein	Antitoxin
ASA_RS23705	331..600	+	270	WP_073531821.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASA_RS00100	709..1338	+	630	WP_005321764.1	hypothetical protein	-
ASA_RS00105	1914..2900	+	987	WP_011116722.1	replication initiation protein	-
ASA_RS00110	3015..3338	-	324	WP_005321770.1	hypothetical protein	-
ASA_RS00115	3549..3959	+	411	WP_005321773.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5616	5616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Relaxosome protein TraY (4-47)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10358.02 Da
• Isoelectric Point: 10.3800

>T20977 WP_073531821.1 NC_004924:331-600 [Aeromonas salmonicida subsp. salmonicida A449]
MAWKVELTDTAKKQLARLDKTQSQRITKYLRRIMMLENPRDAGKALTGNLRTYWRYRVGDYRVVCDIRDNDLVIVAVIIG
HRSEVYGGS

Nucleotide

• Length: 270 bp

>T20977 NC_004924:331-600 [Aeromonas salmonicida subsp. salmonicida A449]
TTGGCTTGGAAAGTTGAATTGACCGATACCGCCAAGAAGCAATTGGCTAGGCTGGATAAGACCCAATCGCAGAGGATCAC
AAAATATCTGCGGCGGATTATGATGCTTGAAAATCCGAGGGATGCTGGCAAAGCTCTAACCGGTAATTTACGAACCTACT
GGCGTTATCGCGTGGGGGATTATCGGGTTGTGTGTGACATAAGAGATAACGATCTTGTCATTGTTGCTGTGATAATTGGG
CATCGCAGTGAAGTATATGGTGGAAGTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8686.77 Da
• Isoelectric Point: 4.2712

>AT20977 WP_005321784.1 NC_004924:123-347 [Aeromonas salmonicida subsp. salmonicida A449]
MLAIRLPSEIEERLANLAQETGRTKTFYAREAILTHLEELEDYYLAERVMENVRKGEERLFSLDDVERDLGLES

Nucleotide

• Length: 225 bp

>AT20977 NC_004924:123-347 [Aeromonas salmonicida subsp. salmonicida A449]
ATGTTAGCGATACGCTTGCCTTCGGAGATCGAAGAGCGTTTGGCAAATTTGGCGCAAGAGACGGGAAGAACGAAGACCTT
CTATGCACGGGAGGCCATCCTGACCCATCTGGAAGAGTTGGAAGATTACTATCTGGCTGAGAGGGTCATGGAGAATGTTC
GCAAGGGCGAAGAGCGCCTGTTCTCATTGGATGACGTGGAGCGCGATCTTGGCTTGGAAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A151KEE9